We offer two variants of the cAMP biosensor, and we recommend the pGloSensor™-22F cAMP Plasmid as the first choice for most applications.
pGloSensor™-22F cAMP Plasmid. Following cell-free expression in vitro, the version encoded by this construct shows an increased EC50 for activation together with increased signal-to-background ratio at cAMP saturation relative to the version encoded by the pGloSensor™-20F cAMP construct. In general, we have observed similar relationships between the two constructs when their performance is compared in living cells.
pGloSensor™-20F cAMP Plasmid. The version encoded by this construct performs well in HEK293 cells at 37°C. Luminescence from the pGloSensor™-22F cAMP Plasmid construct can be more difficult to detect at physiologic temperatures.
For a thorough explanation of the performance differences between the two plasmids, please consult Section 3.B, Recommendations on Choice of GloSensor™ Plasmid, in Technical Manual #TM076 (provided in the Protocol Section below).
Through our partner Addgene, two variants of the cGMP biosensor are available, along with a biosensor designed to monitor caspase-3/7 protease activity.
pGloSensor™-42F cGMP Vector (Addgene Plasmid #236806). This construct is preferred when assaying an endogenous guanylyl cyclase, providing maximal sensitivity for lower intracellular levels of cGMP.
pGloSensor™-40F cGMP Vector (Addgene Plasmid #236812). This version is preferred when overexpressing a guanylyl cyclase, providing maximal dynamic range at high intracellular levels of cGMP.
pGloSensor™-30F DEVD Caspase Vector (Addgene Plasmid #236880). The GloSensor™ 30F protease construct, engineered with a caspase-3/7 cleavage site, provides a sensitive, real-time, non-lytic approach for measuring caspase-3/7 activity in cell culture and in vivo.