The sensitivity of the UMP/CMP-Glo™ Glycosyltransferase Assay means it can detect low UMP or CMP concentrations
with high dynamic range. Therefore, whether assaying for a low-activity glycosyltransferase whose sugar transfer rate is
low or using a low concentration of enzyme that produces a small amount of UMP or CMP, you can obtain a high signal-to-background
ratio using the assay.
The data below show the linearity and sensitivity of the UMP/CMP-Glo™ Glycosyltransferase Assay. Here, a UMP or CMP standard curve was prepared over the indicated concentration range in 25µl of 1X glycosyltransferase reaction buffer in a solid white 96-well plate. UMP/CMP-Glo™ Glycosyltransferase Assay was performed using 25µl of UMP/CMP Detection Reagent at room temperature. Luminescence was recorded using a GloMax® luminometer. Values represent the mean of two replicates. The tables show luminescence measured 1 hour after adding the UMP/
CMP Detection Reagent, and signal-to-background ratios calculated for each concentration of the standard curve.