Lumit® IL-8 (Human) Immunoassay
No-Wash Immunoassay for IL-8
- ​Cell-based assay with fast add-and-read protocol
- Luminescent detection with any plate-reading luminometer
- Scalable to 96- or 384- well plates, protocols included
- Pack sizes show assay number for 96-well plates
Catalog Number:
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$ 665.00
Catalog Number: W1460
$ 4,326.00
Catalog Number: W1461
$ 2,487.00
Catalog Number: W1462
Catalog Number: W146A
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Quantify IL-8 Release Without Tedious Wash Steps
The Lumit® IL-8 Immunoassay quantitatively measures IL-8 secretion in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.
Note: The total number of assays per kit will vary based on final well volume.
For standard well volumes:
- 100 assay kits: applicable for 100 assays in 96-well plates or 400 assays in 384-well plates.
- 500 assay kits: applicable for 500 assays in 96-well plates or 2,000 assays in 384-well plates.
- 1,000 assay kits: applicable for 1,000 assays in 96-well plates or 4,000 assays in 384-well plates.
How the Lumit® IL-8 Immunoassay Works
Primary antibodies to IL-8 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-8, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-8 levels. The assay is specific to IL-8 and detects both 72 and 77 amino acid major isoforms.
Simple One-Plate Protocol Requires No Wash Steps
High Sensitivity of Lumit® IL-8 Immunoassay
The sub-picomolar to nanomolar linear detection range of the Lumit® IL-8 Immunoassay covers expected concentrations for most cell models.
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Specification |
Lumit® IL-8 Immunoassay |
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Dynamic Range |
7.29–10,000pg/ml |
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Limit of Detection (LOD) |
1.7pg/ml (3 SD above background) |
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Minimal Detectable Dose (MDD) |
1.6pg/ml (2 SD above background) |
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Assay Time |
70 minutes |
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Sample Type |
Cell culture supernatants |
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SD=Standard Deviation
High-Throughput Screening Applications
Optimizing the Lumit® IL-6 Immunoassay for High-Throughput Drug Screening
In this webinar, we share key considerations for scaling Lumit® Immunoassays from a single 96-well plate to many 364-well plates and offer tips for optimizing Lumit® Immunoassays for HTS applications. You will also hear Galapagos scientist Dr. Nick Verstraeten share how his team used the Lumit® IL-6 Immunoassay in a screening campaign for antiviral drugs.
Lumit® Cytokine Assay Automation for High-Throughput Screening
In this poster, see miniaturization and automation data for Lumit® cytokine assays.
Sensitive Detection of IL-8 from THP-1 Cells
Human THP-1 cells (10,000/well in 96-well plates) were treated for 22 hours with increasing concentrations of LPS, with or without IFN-γ. After treatment, Lumit® IL-8 assay was used to quantitate IL-8 released from cells.
For additional examples of Lumit® IL-8 detection from cells, including miniaturization to 384-well plates, check out this scientific poster: Homogeneous bioluminescent IL-8 and MCP-1 immunoassays detect profound synergistic regulation of release from human monocytes
For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.
Jonathan Chow, Principal Scientist, Corner Therapeutics
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml |
Anti-hIL-8 mAb-SmBiT, 500X |
W147A | 1 × 30μl |
Anti-hIL-8 mAb-LgBiT, 500X |
W148A | 1 × 30μl |
Human IL-8 Standard |
W146A | 1 × 25μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml |
Anti-hIL-8 mAb-SmBiT, 500X |
W147B | 1 × 300μl |
Anti-hIL-8 mAb-LgBiT, 500X |
W148B | 1 × 300μl |
Human IL-8 Standard |
W146A | 1 × 25μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml |
Anti-hIL-8 mAb-SmBiT, 500X |
W147A | 5 × 30μl |
Anti-hIL-8 mAb-LgBiT, 500X |
W148A | 5 × 30μl |
Human IL-8 Standard |
W146A | 1 × 25μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Human IL-8 Standard |
W146A | 1 × 25μl |
Resources
Articles
Posters
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