Unmethylated cl857 Sam7 Lambda DNA (48,502bp) is isolated from infected GM119, an E. coli strain lacking both the dam and dcm methylase activities. Unmethylated Lambda DNA is used as a substrate for restriction enzymes sensitive to DNA methylation.
Storage Buffer: 10mM Tris-HCl (pH 7.5), 10mM NaCl, 1mM EDTA.
GenBank - EMBL Accession Number: J02459.
- Arraj, J.A. and Marinus, M.G. (1983) J. Bacteriol. 153, 562–5.