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L-Rhamnose Monohydrate

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Induces Expression of ORFs in Vectors Transformed into Single Step (KRX) E. coli Cells

  • Used with Single Step (KRX) Competent Cells
  • Provides dramatic control of expressed protein-coding regions with higher protein yields
  • Induces expression of T7 RNA Polymerase under control of rhamnose promoter rhaBAD

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Catalog number selected: L5701

$ 52.00
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L-Rhamnose Monohydrate
10g
$ 52.00
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The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression. These cells consolidate the best attributes of these two steps into one strain to evaluate protein expression in E. coli. In two days, you can transform your vector into the Single Step (KRX) Competent Cells and be ready for protein expression. Transformation efficiencies are greater than 108 cfu/μg, similar to other highly competent cells. The single step cells are available in single transformation size (50μl). KRX also can be used for blue/white screening. Single Step (KRX) is an E. coli K strain that contains a chromosomal copy of the T7 RNA polymerase driven by a rhamnose promoter (rhaBAD) to provide dramatic control of the proteins expressed via a T7 promoter. Pre-induced expression protein levels are significantly lower than those of BL21(DE3)-derived strains. This feature facilitates cloning and expression of proteins toxic to E. coli.

Genotype: [F´, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk, mk+), e14 (McrA), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: L5702 Change Configuration

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

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