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BCIP/NBT Color Development Substrate
(5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium)

S3771

  • Quality tested and qualified for use in blotting

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Catalog number selected: S3771

$ 78.00 Your price: Log In

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BCIP/NBT Color Development Substrate
(5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium)
1.25/2.5ml
$ 78.00
Your price: Log In

BCIP (5-bromo-4-chloro-3-indolyl-phosphate) is used in conjunction with NBT (nitro blue tetrazolium) for the colorimetric detection of alkaline phosphatase activity. Each vial of BCIP is supplied with a vial of NBT.

Preparation of Substrates to Detect Alkaline Phosphatase: For every 5ml of alkaline phosphatase buffer (100mM Tris-HCl [pH 9.0], 150mM NaCl, 1mM MgCl2), add 33μl NBT and 16.5μl BCIP. Add the NBT first, mix, add the BCIP, and mix again. Use within 1 hour, and discard any unused solution.

Concentration: BCIP (50mg/ml) in 100% dimethylformamide; NBT (50mg/ml) in 70% dimethylformamide.

Specifications

What's in the box?

Item Part # Size

NBT

S380C 1 × 2.5ml

BCIP

S381C 1 × 1.25ml

SDS

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

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