QuantiFluor® RNA System
Sensitive Quantitation of Small Amounts of RNA in Solution
- Requires less template RNA than other systems
- More sensitive than absorbance methods for low-concentration samples
Catalog Number:
Size
Highly Sensitive, Easy-to-Use, Fluorescent Dye for RNA Quantitation
The QuantiFluor® RNA System contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution. Detecting and quantitating small amounts of RNA is important for many biological applications, including determining yield of in vitro transcribed RNA and measuring RNA concentration before Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, RT-PCR and differential display PCR.
The QuantiFluor® System requires less RNA (100pg) than other RNA quantitation methods (~1µg), so you save more of your sample for downstream assays.
Improved Sensitivity vs. Absorbance and Other Fluorescent RNA Quantitation Systems
The system is designed to measure 0.1–500ng/µl RNA (from 1µl sample in a 200µl assay) and offers significantly increased sensitivity over absorbance at 260nm (NanoDrop) for samples containing low concentrations of RNA. Sensitivity is equivalent to that of Quant-iT™ RiboGreen®, with wider dynamic range.
| Sample* | Assay | |
|---|---|---|
|
QuantiFluor® RNA System
|
0.1–500ng/µl
|
0.1–500ng
|
|
Quant-iT™ RiboGreen
|
0.1–200ng/µl
|
0.1–200ng
|
|
Qubit® RNA HS (5–100ng)
|
5–100ng/µl
|
5–100ng
|
|
NanoDrop® 2000 Spectrophotometer
|
2–12,000ng/µl
|
2–12,000ng
|
RNA Quantitation Sensitivity and Assay Linearity
The QuantiFluor® RNA System detects as little as 0.5ng/ml RNA in a 96-well microplate assay (corresponding to 1µl of a 100ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.
Easy Setup on Microplate or Single-Tube Fluorometers
The QuantiFluor® RNA System includes all the necessary reagents to quickly set up and quantitate RNA, and is easy to set up on microplate or single-tube fluorometers. The System is compatible with any fluorometer capable of measuring the appropriate fluorescence excitation and emission spectra. Compatible Promega fluorometers include:
How Do Fluorescent Dyes Compare to Other Quantitation Methods?
In these short videos, Promega Applications Scientist Doug Wieczorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
UV-Absorbance vs Fluorescence
Fluorescence vs qPCR
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
20X TE Buffer (pH 7.5) |
E260A | 1 × 25ml | View Product |
|
RNA Standard |
E299A | 1 × 100μg | |
QuantiFluor® RNA Dye |
E286A | 1 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
Resources
Articles
- RNA Isolation from 3D Microtissue Cultures: A Comparison of Manual and Automated Methods
- microRNA Purification from Plant Tissues Using the ReliaPrep™ miRNA Cell and Tissue Miniprep System
- Measuring the QuantiFluor™ RNA System Using the Qubit® 2.0 Fluorometer
- Measuring the QuantiFluor™ RNA System Using the QuantiFluor™-ST Fluorometer
- Total RNA Isolation from 3D Cell Cultures or Cells in Matrigel® Matrix Using the ReliaPrep™ miRNA Cell and Tissue Miniprep System
- Establishment of preanalytical conditions for microRNA profile analysis of clinical plasma samples
Software and Firmware
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