TaqI
- Restriction endonuclease from Thermus aquaticus YTI
Catalog Number:
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Size
Cut Site:
Tâ–¼CG Â A
AÂ GCâ–²T
Note: Sensitive to overlapping dam methylation.
Incubation Conditions: Buffer E. 65°C. (TaqI exhibits <10% activity at 37°C.)
Source: Thermus aquaticus YTI.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | E | MULTI-COREâ„¢ |
|---|---|---|---|---|---|
| 10–25% | 25–50% | 50–75% | 50–75% | 100% | 100% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 121 | 50 | 10 | 4 | 13 | 7 |
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer E 10X Buffer |
R005A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
TaqI |
R615A | 1 × 1,000u | ||
|
MULTI-COREâ„¢ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer E 10X Buffer |
R005A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
TaqI |
R615E | 1 × 10,000u | ||
|
MULTI-COREâ„¢ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Other Resources
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Thirteen fragments of 125–21,226bp generated from Lambda DNA digested with EcoRI and HindIII.
G1731
