Transcend™ Non-Radioactive Translation Detection System Technical Bulletin

Instructions for Use of Product(s)
L5061, L5070

Literature # TB182

The Transcend™ Non-Radioactive Translation Detection Systems allow non-radioactive detection of proteins synthesized in vitro. Using these systems, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [35S]methionine or other radioactive amino acids. This biotinylated lysine is added to the translation reaction as a precharged, ε-labeled biotinylated lysine-tRNA complex (Transcend™ tRNA) rather than a free amino acid. After SDS-PAGE and electroblotting, the biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish Peroxidase (Streptavidin-HRP), followed either by colorimetric or chemiluminescent detection. Typically, 0.5–5ng of protein can be detected by these methods within 3–4 hours after gel electrophoresis.

Summary of Changes
The following changes were made to the 8/21 revision of this document:
1. Removed discontinued Cat.# L5080 and associated chemiluminescent detection instructions (Section 5.D).
2. Updated Sections 1 and 6, including Figure 2.
3. Moved to a new template.

Revised 8/21.

Experienced User Protocols