Transcend™ Non-Radioactive Translation Detection System
Non-Radioactive Detection of In Vitro Translated Proteins
- Biotin tag allows detection of 0.5–5ng of protein 3–4 hours after gel electrophoresis
- No radioisotope handling, storage or disposal issues
Catalog Number:
Size
Catalog Number: L5070
Non-radioactive detection of proteins synthesized in vitro. Using this system, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [35S]methionine or other radioactive amino acids. Biotinylated lysine is added to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA complex (Transcend™ tRNA) rather than as a free amino acid. After SDS-PAGE and electroblotting, biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish Peroxidase (Streptavidin-HRP), followed by colorimetric detection. Typically, this system enables you to detect 0.5–5ng of protein in 3–4 hours after gel electrophoresis. This sensitivity is equivalent to that achieved with [35S]methionine incorporation and autoradiographic detection 6–12 hours after gel electrophoresis.
For more information, see the Protocols & Applications Guide.
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Transcend™ tRNA |
L506A | 1 × 30μl | View Product |
|
Western Blue® Stabilized Substrate |
S384C | 1 × 35ml | View Product |
|
Streptavidin Alkaline Phosphatase |
V559B | 1 × 36μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
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