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PureYield™ Plasmid Midiprep System

Up to 200µg of Transfection-Ready Plasmid DNA from 50ml Cultures

  • 30-minute, vacuum-based protocol
  • Highly pure, concentrated plasmid DNA
  • No tedious, gravity-drip columns or post-elution alcohol precipitation

Size

Catalog number selected: A2492

$ 272.00
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This product is discontinued
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PureYield™ Plasmid Midiprep System
25 preps
$ 272.00
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The PureYield™ Plasmid Midiprep System provides a rapid method for purification of 100–200μg of transfection-quality plasmid DNA from 50ml bacteria cultures. Using the vacuum protocol, plasmid isolation is complete in as little as 30 minutes, significantly reducing time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.

The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves performance in sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation.

PureYield™ Plasmid Midipreps can be used with centrifugation or vacuum-based protocols. 

Proper assembly of Lysate Clearing Column (blue) and DNA Binding Column (white) for use with the PureYield™ Plasmid Midiprep System DNA Purification by Vacuum protocol.

Proper assembly of Lysate Clearing Column (blue) and DNA Binding Column (white) for use with the PureYield™ Plasmid Midiprep System DNA Purification by Vacuum protocol.
Proper assembly of Lysate Clearing Column (blue) and DNA Binding Column (white) for use with the PureYield™ Plasmid Midiprep System DNA Purification by Vacuum protocol.

Specifications

You are viewing: A2492 Change Configuration

What's in the box?

Item Part # Size Available Separately

Neutralization Solution (NSB)

A148H 1 × 130ml View Product

PureYield™ Binding Columns

A245A 5 × 5 each

PureYield™ Clearing Columns

A246A 5 × 5 each

Endotoxin Removal Wash

A251C 1 × 85.3ml

Column Wash

A252C 1 × 210ml

Cell Resuspension Solution (CRA)

A711F 1 × 75ml View Product

Cell Lysis Solution (CLA)

A712F 1 × 75ml View Product

Nuclease-Free Water

P119C 1 × 25ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: A2495 Change Configuration

What's in the box?

Item Part # Size Available Separately

Neutralization Solution (NSB)

A1485 1 × 500ml

PureYield™ Binding Columns

A245A 20 × 5 each

PureYield™ Clearing Columns

A246A 20 × 5 each

Endotoxin Removal Wash

A251B 1 × 315ml

Column Wash

A252B 2 × 381ml

Cell Resuspension Solution (CRA)

A7115 1 × 315ml

Cell Lysis Solution (CLA)

A7125 1 × 315ml

Nuclease-Free Water

P119C 4 × 25ml View Product

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: A2496 Change Configuration

What's in the box?

Item Part # Size Available Separately

Neutralization Solution (NSB)

A1485 3 × 500ml

PureYield™ Binding Columns

A245E 3 × 100 each

PureYield™ Clearing Columns

A246E 3 × 100 each

Endotoxin Removal Wash

A251B 3 × 315ml

Column Wash

A252B 6 × 381ml

Cell Resuspension Solution (CRA)

A7115 3 × 315ml

Cell Lysis Solution (CLA)

A7125 3 × 315ml

Nuclease-Free Water

P1195 2 × 150ml View Product

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

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