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Tools to Improve Protein Sample Preparation for Mass Spec

Prior to joining industry Dr. Saveliev worked on various academic projects including epigenomics, DNA repair and recombination, mechanisms of radioresistance and circadian rhythm. Dr. Saveliev’s research efforts were supported by US government funding, and the results were published in the leading academic journals such as the EMBO Journal, PNAS, PLOS, and Genes & Development.

After joining Promega Corporation in 2004, Dr. Saveliev utilized his expertise to develop tools for life sciences. Lately, his product development efforts have been focused on tools for protein mass spectrometry sample preparation. Sergei has played the key role in building mass spectrometry protein analytical portfolio at Promega. His efforts are directed toward the development of solutions that help overcome the major challenges in protein mass spec sample analysis including efficient proteolysis, standardization, suppressing of side effects of protein mass spec sample preparation and others.

  • Sergei Saveliev, PhD

  • Sr. Research Scientist

  • Original Webinar Date: Tuesday, May 24, 2016

This webinar will discuss common shortcomings of protein mass spec sample preparation and provide solutions to these shortcomings.

Common shortcomings of protein mass spec sample preparation include incomplete digestion, lengthy and laborious procedure, low protein sequence coverage, inefficient peptide recovery of in-gel digested proteins or poor protein extraction from cells and tissues and inadequate instrument performance monitoring. In this webinar, we will offer solutions to these shortcomings. We will discuss the advantages of trypsin preparations with enhanced proteolytic efficiency. We will also review mass spec compatible SDS surfactant analogs and their advantage for in-gel protein digestion, peptide recovery and protein extraction from tissues. The discussion will involve a case study showing positive impact of proteases with alternative cleavage specificity for increasing of sequence coverage of analyzed proteins. In the final part, we will review the advantages of standardized total cell protein extracts and a mix of heavy isotope labeled peptide standards for optimization of protein mass spec sample preparation and monitoring of key performance parameters of mass spec instruments.