Troubleshooting Cell-based Assays Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis

Dr. Jessica Wagener is Application Specialist focusing on Cell Handling at Eppendorf Headquarter in Hamburg, Germany. Entering a cell culture laboratory as a graduate student for the first time in 2006, she came to Eppendorf with 5+ years of experience in cell culture. After studying Biology at the Universities of Marburg and Düsseldorf, she investigated apoptosis mechanisms in different cell lines for her diploma thesis. Following one year as Research Associate working with primary cells she started to work towards her PhD with a focus on reproductive biology at the Medical School Essen. She joined Eppendorf in April 2012 shortly after completing her PhD in Biology.

Erik Bonke received a Diploma in biology from the Johannes-Gutenberg University in Mainz, Germany in 2012. In his diploma thesis at the University Hospital in Mainz he worked at the genetic manipulation of murine embryonic stem cells in order to generate different transgenic mouse strains. He did his doctoral thesis at the University Hospital in Frankfurt am Main, where he focused on the mechanistic fundamentals of mitochondrial ROS generation and their physiological implication as cellular second messenger molecules, a process termed redox signaling. After completion of the experimental part of his thesis, Mr. Bonke joined Promega Germany to work as an Application Specialist with a main focus on cellular reporter technologies. As part of this position, he is giving frequent practical as well as theoretical workshops/seminars on the application of Promega's current luminescent reporter portfolio. In 2018, he was awarded a doctoral degree by the Johann Wolfgang Goethe University Frankfurt.