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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

How to Integrate Cellular Metabolism Assays Into Your Research: Considerations and Challenges

Cell-Based Assays

Tuesday, September 12, 2017

Donna Leippe, PhD

Metabolite detection technologies can pose challenges such as laborious sample preparation and the inability to assay numerous samples rapidly. Additionally, lack of sensitivity and dynamic range prevents detection of metabolites directly in cells in multi-well plates. In this webinar, we describe new bioluminescent metabolite assays and sample processing strategies that enable rapid measurements of key energy metabolites in plate formats and show how the assays can be used to study cancer cell metabolism and activation of T cells.

Bioactivity Assays: Putting the Puzzle Together

Drug Discovery

Tuesday, September 19, 2017

Ulrike Herbrand, PhD

In past years, the requirement for bioactivity testing changed from later phase lot release and stability testing to additional applications like biocomparability testing for follow-on biologics, accelerated stress condition testing and confirmation of successful production scale up. Therefore a suitable bioassay is needed at a very early time point. This webinar will look at a wide variety of therapeutic proteins and illustrate some best practices in method development that results in reliable, reproducible and precise assays, and discuss which assays may be best for your product and development program.

Applications of a Bioluminescent Peptide Tag: Simple, Quantitative Protein Detection Down to Endogenous Levels

Cell-Based Assays

Tuesday, September 26, 2017

Christopher Eggers, PhD

Regulated protein abundance and surface expression are fundamental to normal cellular physiology and dysregulation underlies many disease states. Monitoring changes in protein abundance usually involves labor-intensive, antibody-based methods that yield only semi-quantitative results. This webinar will introduce a bioluminescent tagging system that can quantify proteins in minutes using simple, antibody-free methods. This system offers wide dynamic range and sensitivity compatible with detecting even endogenously expressed proteins when combined with CRISPR gene editing.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

Understanding Luminescent Reporter Assay Design

Cell-Based Assays

Tuesday, March 08, 2016

Carl Strayer, PhD

Luminescent reporter assays are powerful research tools for a variety of applications. This webinar explains critical aspects of reporter assay design to help you implement a robust assay that delivers meaningful results.

Introduction to Nucleic Acid Purification: Purification Basics and Their Application to Different Sample Types

Genomics

Tuesday, February 09, 2016

Paraj Mandrekar

Nucleic acid purification is the first step in genomics research. Come learn about the basics of nucleic acid purification, approaches taken for purification of DNA or RNA and how purification chemistries are applied to common nucleic acid purification workflows.

Maintaining Scientific Integrity Through Cell Line Authentication

Genomics

Tuesday, January 26, 2016

Gabriela Saldanha & Martin Ensenberger, PhD

Human cell line misidentification is a common problem in the research community that can have significant negative consequences. As of January 2016, applicants for grants will be required to authenticate key biological resources, including cell lines. This webinar will provide a brief history of cell line contamination and outline a simple and inexpensive method for authenticating human cell lines.

Understanding the Role of the Inflammasome in Inflammation

Cell-Based Assays

Tuesday, January 12, 2016

Martha O'Brien, PhD

This webinar will discuss two important outcomes of inflammasome induction, caspase-1 activation and pyroptosis, and will present a new method for directly measuring caspase-1 activation.

Methodologies and Considerations for Quantitation in Next Generation Sequencing Library Preparation

Genomics

Tuesday, December 15, 2015

Adam Blatter & Curtis Knox

A review of technologies for DNA quantitation before and after NGS library preparation.

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