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Home » Resources »

Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

Biologics Forum: Tips and Tools for Successful Immunotherapy Bioassay Development

Drug Discovery

Tuesday, April 23, 2019

Zhijie Jey Cheng, PhD, Manuela Grassi, PhD and Richard Somberg, PhD

Mechanism of action (MOA)-based bioassays are a critical component of biologics drug development. However, establishing a functional bioassay that meets the regulatory guidelines for use in potency determination and lot release can be challenging. Join our panel of bioassay experts for a live discussion on bioassay design and selection, assay optimization, and bioassay qualification for potency lot release.

The Y-HRD Database: How It Works and How to Use It in Casework

Genetic Identity

Wednesday, May 08, 2019

Lutz Roewer, PhD

The Y-Chromosome Haplotype Reference Database (Y-HRD) includes Y-chromosome haplotype data from 135 national databases populated over the past 20 years. Y-based ancestry prediction and familial searching can provide important investigative leads. This webinar will discuss how to use the Y-HRD to facilitate statistical analyses for a range of casework applications and interpretation purposes (e.g., frequency estimation, kinship and mixture analysis and ancestry prediction).

Developing More Efficient Biologic Workflows with Automated Plasmid Preps and Protein/Antibody Purification

Genomics

Tuesday, May 14, 2019

Dr. Nidhi Nath, Doug White and Chris D'Jamoos

Development and screening of biologics can be a time-consuming process due to the volume of samples processed for cloning, isolation of plasmids and purification of candidate antibodies. This webinar will discuss how our field-based automation team can help you develop walk-away automation solutions to speed up your purification workflow.

Studying Endogeneous Protein Dynamics with CRISPR-Mediated Tagging: Understanding Your Options

Cell-Based Assays

Tuesday, June 04, 2019

Christopher Eggers, PhD

An exciting application of CRISPR/Cas9-mediated gene editing is the ability to tag and measure endogenously expressed proteins while maintaining native expression levels and regulation. In this webinar, we will discuss available options for simple and efficient knock-in of a bioluminescent peptide tag to enable sensitive, homogeneous assays for measuring protein dynamics.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

Improved Chemistry for NGS Library Cleanup and Size Selection

Genomics

Tuesday, October 10, 2017

Charles Cowles, PhD

Current size-selective DNA purification methods need improvement with respect to sample loss, poor reproducibility, high viscosity and recovery of undesired high MW fragments. With new size selection chemistry, you can improve yield, selectivity, usability, and functionality in NGS library cleanup and size selection, as well as PCR cleanup and other applications.

Applications of a Bioluminescent Peptide Tag: Simple, Quantitative Protein Detection Down to Endogenous Levels

Cell-Based Assays

Tuesday, September 26, 2017

Christopher Eggers, PhD

Regulated protein abundance and surface expression are fundamental to normal cellular physiology and dysregulation underlies many disease states. Monitoring changes in protein abundance usually involves labor-intensive, antibody-based methods that yield only semi-quantitative results. This webinar will introduce a bioluminescent tagging system that can quantify proteins in minutes using simple, antibody-free methods. This system offers wide dynamic range and sensitivity compatible with detecting even endogenously expressed proteins when combined with CRISPR gene editing.

Bioactivity Assays: Putting the Puzzle Together

Drug Discovery

Tuesday, September 19, 2017

Ulrike Herbrand, PhD

In past years, the requirement for bioactivity testing changed from later phase lot release and stability testing to additional applications like biocomparability testing for follow-on biologics, accelerated stress condition testing and confirmation of successful production scale up. Therefore a suitable bioassay is needed at a very early time point. This webinar will look at a wide variety of therapeutic proteins and illustrate some best practices in method development that results in reliable, reproducible and precise assays, and discuss which assays may be best for your product and development program.

How to Integrate Cellular Metabolism Assays Into Your Research: Considerations and Challenges

Cell-Based Assays

Tuesday, September 12, 2017

Donna Leippe, PhD

Metabolite detection technologies can pose challenges such as laborious sample preparation and the inability to assay numerous samples rapidly. Additionally, lack of sensitivity and dynamic range prevents detection of metabolites directly in cells in multi-well plates. In this webinar, we describe new bioluminescent metabolite assays and sample processing strategies that enable rapid measurements of key energy metabolites in plate formats and show how the assays can be used to study cancer cell metabolism and activation of T cells.

How to Avoid Artificial Non-Enzymatic PTMs During the Peptide Sample Preparation Process

Proteomics

Tuesday, June 13, 2017

Sergei Saveliev, PhD

Non-enzymatic post-translational modifications (PTMs) spontaneously occur in biotherapeutic proteins during manufacturing and storage. These modifications negatively affect efficacy and stability of biotherapeutic proteins. Major non-enzymatic PTMs are deamidation, disulfide bond scrambling and oxidation. These non-enzymatic PTMs are also introduced during protein preparation for peptide mapping and compromise the analysis. In the webinar recording Dr. Saveliev discusses sources of these artificial protein modifications as well as procedural optimizations to suppress these PTMs.

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