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Tuesday, March 14, 2017
Terry Riss, PhD
3D cell culture models present a challenge for using assay chemistries and imaging methods that were originally designed for measuring events from 2D monolayers of cells. In this webinar, we will present factors to consider when designing and optimizing assays applied to 3D cell culture models, case studies describing design of reagents to overcome the limitations inherent with detecting markers from large 3D culture models, and applications of novel real-time detection methods.
Tuesday, February 28, 2017
Brad Hook, PhD
MicroRNAs (miRNAs) are small-noncoding RNA molecules that regulate gene expression post-transcriptionally. Amplification and overexpression of individual oncomiRs (miRNAs associated with cancer) or genetic loss of tumor suppressor miRNAs are associated with human cancer and are sufficient to drive tumorigenesis in mouse models. A global decrease in miRNA levels has been observed in human cancers and linked to genetic and epigenetic alterations in components of the miRNA biogenesis machinery. Learn more about how to analyze miRNAs in human cancer cells as we present data on a two studies that use new novel assays to monitor cell health and purify total RNA (including miRNAs) from the same cells.
Tuesday, February 14, 2017
Doug Wieczorek, PhD
Success or failure in DNA/RNA analysis applications often comes down to whether or not the appropriate amount of input nucleic acid is used, but nucleic acid quantitation methods reveal different information about a sample. This webinar will review absorbance, fluorescent nucleic acid-binding dyes and qPCR quantitation methods and present the advantages and disadvantages of each method. With this information, you'll be able to choose the appropriate quantitation method based on your sample type and downstream application.
Tuesday, January 24, 2017
Dr. William Rehrauer
Learn about the detection of novel RNA fusions via new next generation sequencing library prep methods, automated extraction of total nucleic acid and the utility of this research in the field of molecular oncology.
Tuesday, January 10, 2017
Danette L Daniels, PhD
Modification of gene expression rather than alteration of the genetic code (Epigenetics) is an interesting way that organisms develop a phenotype. Altered expression of the key proteins can have deleterious effects. Researchers are actively engaged in identifying modulators of epigenetic proteins and learning how these proteins interact with their targets. Learn more as we present data on the use of a novel BRET assay for such studies.