Notes: The TNT® Coupled Reticulocyte System and Canine Pancreatic Microsomal Membranes (CMMs) were used to express a clone isolated through a differential display screen. (1291)

Notes: The pGEM^®-T Vector System was used to clone the products from PCR differential display. Sequencing of the insert was performed. (1550)

Notes: The MTS-based CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay was used to measure viability of rat glial cells treated with antisense oligodeoxynucleotide. (1539)

Notes: [³⁵S]methionine-labeled pre-IL-1β and PARP(T) proteins were synthesized using the TNT^® T7 Coupled Reticulocyte Lysate System and were used for cleavage studies. The proteins were incubated with purified recombinant human ICE, which cleaved both proteins. The cleavage of PARP requires 50- to 100-fold higher concentrations of ICE than required for pre-IL-1β cleavage. (1060)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from CD4+CD8+ thymocytes. The isolated RNA was used for RT-PCR. (1663)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from CD4+CD8+ thymocytes. The isolated RNA was used for RT-PCR. (0291)

Notes: The PolyATtract^® System 1000 was used to isolate poly A+ RNA directly from the adrenal glands of rats treated with reserpine and from nontreated controls. The isolated poly A+ RNA was used for differential display RT-PCR (1652)

Notes: Total RNA was isolated from the human malaria parasite, Plasmodium falciparum, using the RNAgents^® Assay System. The isolated RNA was used in RT-PCR. (0708)

Notes: RNA was isolated from the human malaria parasite, Plasmodium falciparum using the RNAgents^® Total RNA Isolation System. The isolated RNA was used for RT-PCR. (1660)

Notes: PCR products were cloned into pGEM^®-T Vector System. (0250)

Notes: Poly A+ RNA was isolated from the protozoan Leishmania mexicanna amazonesis total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM-T Vector. (1678)

Notes: PolyATtract^® mRNA Isolation System was used to isolate Poly A+ RNA from the protozoan Leishmania mexicanna amazonesis total RNA. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM^®-T Vector. (0748)

Notes: The inhibitor was used to protect RNA during RT-PCR. (2246)

Notes: PolyA+ RNA was isolated from J2E erythroid cell total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for RT-PCR. (1691)

Notes: The PolyATtract^® System 1000 was used to isolate polyA+ RNA from Pisium sp. plant tissue. Many details of the isolation are given in the paper. The isolated polyA+ RNA was used for RT-PCR and Northern blot analyses. (0736)

Notes: The system was used to isolate poly A+ RNA for INS-1 cell total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used in RT-PCR and Northern analysis. (1700)

Notes: PCR products of 372bp and 426bp were subcloned into the pGEM^®-T Vector and used to produce RNA probes for Northern blotting and in situ hybridization. (1552)

Notes: Superior cervical ganglia were maintained in culture with or without the 2.5S NGF. RNA was isolated from these cultures and used for differential display with RT-PCR. The RT-PCR products were cloned with the pGEM^®-T Vector System. (0998)

Notes: Point mutations in the repeated portion of the PKD1 gene were detected by the Protein Truncation Test. PCR products containing the T7 promoter were used as templates and protein was labeled by incorporation of [³H] leucine in a transcription/translation reaction using TNT^® Coupled Reticulocyte Lysate System. The pGEM^®-T Vector System was also used. (0489)

Notes: PolyA⁺ RNA was isolated from rat total RNA using the PolyATtract^® mRNA Isolation System and used for Northern analysis. The pGEM^®-T Vector System was used to clone products from RT-PCR. (0447)

Notes: Poly(A+) RNA was isolated from total RNA extracted from T47D mammary carcinoma cell line using the PolyATtract^® mRNA Isolation System. The RNA was used for northern blots. RQ1 was used to digest genomic DNA away from total RNA. The pGEM®-T Vector was used to subclone PCR products generated by differential display. (1673)

Notes: The inhibitor was used to protect RNA during RT-PCR. 125I-NGF was produced and used to crosslink to cell surface proteins on PC12 cells. The NGF was also used to assay for neurite outgrowth of PC12 and two clonal variants. Cells were treated with NGF and nuclear extracts made and used to assay for AP1 binding activity. Tyrosine phosphorylation of PI kinase in response to NGF was assayed. Also, the concentration-dependent effect of NGF on the accumulation of 3H-inositol phosphate was examined in the PC12 cells. The bFGF was used to assay the induction of cell differentiation in the PC12 cell and a clonal variant. (1632)

Notes: DNA with unmethylated cytosines converted to uracil by bisulfite treatment was purified with the Wizard^® PCR Preps DNA Purification System. The DNA was used for methylation-specific PCR. (0149)

Notes: The Access RT-PCR System was used in this study. (1998)

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM^®-T Vector and sequenced. (1491)