Notes: The Access RT-PCR System was used in this study. (1989)

Notes: The Access RT-PCR System was used to amplify the FasL message from total RNA isolated from mice injected with a recombinant adenovirus. The Access RT-PCR System was used as a means to monitor the success of a gene therapy system. The products were analyzed by EtBr-staining and Southern blotting. (0088)

Notes: This article describes the use of the TNT^® T7 Quick Coupled Transcription/Translation System in a process called the ‘ARM’ technique where antibody-ribosome-mRNA (ARM) complexes are selected by protein binding. The ARM complexes are generated during translation of mRNAs that lack stop codons. The functional antibody that is still complexed with the ribosome and mRNA is selected by protein-coupled magnetic beads, and the associated mRNA is reverse-transcribed and amplified by polymerase chain reaction (RT-PCR). The PCR products are then used in subsequent cycles of the method. (2016)

Notes: Taq DNA Polymerase was used for PCR. The PCR products were purified with the Wizard^® PCR Preps DNA Purification System and cloned with the aid of the pGEM^®-T Vector System. (0624)

Notes: The Access RT-PCR System was used in this study. (1994)

Notes: RT-PCR was performed with degenerate primers and the resulting 110bp product was subcloned with the pGEM®-T Vector System. The 110bp fragment was used to screen a cDNA library and four positive plaques were identified. (0650)

Notes: Tryptic peptides, produced with Sequencing Grade Modified Trypsin, of the purified lipase were used to generate primers for RT-PCR. The largest amplimer was purified and used to screen a lambda gt11 library. The isolated 303 amino acid clone and site-specific mutants were put into the pCI-neo Mammalian Expression Vector and expressed in COS cells. The transiently expressed proteins were assayed for esterase and lipase activity. (0960)

Notes: The ribonuclease inhibitor was used to protect RNA during the reverse transcriptase reaction for RT-PCR. The RNasin® Ribonuclease Inhibitor is also used in the RiboMAX® System for RNA stabilization during in vitro transcription. Transcripts were produce with the RiboMAX® System and expresed in Rabbit Lysate with or without Canine Microsomal Membranes. Proteinase K protection assays are performed with proteins expressed in the presence of the membranes with and without Triton X-100 solubilization of the membranes. The proteinase K digests were stopped by the addition of PMSF and immediately loading the sample into 100°C SDS Sample buffer. (1670)

Notes: The RNasin^® Ribonuclease Inhibitor and the pGEM^®-T Vector System were used in this study. The inhibitor was used to protect RNA during the T4 RNA ligase step for 5' RACE analysis. (1643)

Notes: The PolyATtract^® mRNA Isolation System was used to isolate poly A+ RNA from Anopheles gambiae (mosquito) total RNA. The isolated RNA was used for cDNA synthesis with the Universal Riboclone System. (1683)

Notes: A portion of the cytochrome oxidase subunit I cDNA was amplified and cloned into the pGEM^®-T Vector. RNA probes were produced from the pGEM^®-T Vector for in situ hybridization studies. (1547)

Notes: NGF was used to induce PC12 cell differentiation 3-5 days prior to assay. PCR amplimers representing various subunits of the acetylcholine receptor were subcloned into the pGEM^®-T Vector. The resulting clones were used to produce RNA probes for RNase protection assays. Also, a chimeric a5/7-HT3 receptor was generated by PCR, subcloned into the pGEM^®-T Vector and sequenced. (1419)

Notes: PCR products were directly sequenced with the fmol^® DNA Cycle Sequencing System using the end-labeled primer protocol. (0739)

Notes: Promega's Taq DNA Polymerase was used in this study. (1985)

Notes: Promega's Saccharomyces cerevisiae genomic DNA was used as the template for PCR amplification of several genes. (1161)

Notes: Promega's AMV Reverse Transcriptase and Taq DNA Polymerase were used to perform RT-PCR in this study. (2021)

Notes: Taq DNA Polymerase was used in RT-PCR reactions and the products examined by Southern hybridization. Promising bands were cut from the agarose gel and cloned with the pGEM^®-T Vector System. (0529)

Notes: The Access RT-PCR System was used in this study. (1988)

Notes: The authors use the Reverse Transcription System, Wizard^® PCR Preps DNA Purification Systems, Wizard^® Plus Minipreps DNA Purification System and the pGEM^®-T Vector System in their studies. (0445)

Notes: The Access RT-PCR System was used to monitor the expression of the type II iodothyronine deiodinase in primary rat astrocyte cultures following forskolin treatment. (1592)

Notes: PCR products generated by differential display analysis were subcloned into the pGEM^®-T Vector System and subsequently sequenced. (0240)

Notes: The pTargeT™ Vector was used to subclone the 2.5kb Na+/H+ Exchanger 2 and the 2.7kb Na+/H+ Exchanger 3 directly from PCR reactions. The constructs were stably transfected into PS120 Chinese hamster lung fibroblasts and selected for resistance to the neomycin analog, G-418. After 7-10 days of selection, the cells were further tested for expression by RT-PCR and immunoblotting. (1318)

Notes: The Altered Sites^® II in vitro Mutagenesis System was used to introduce four individual amino acid changes into the subtilisin protein. The Wizard^® PCR Preps DNA Purification System was used as well. (0102)

Notes: Glioma cell lines derived from glioblastoma tumors were rapidly screened for premature stop codon or frameshift mutations in the PTEN mRNA by the protein truncation test (PTT). PTT templates were generated by RT-PCR with a T7 RNA polymerase transcription site in the upstream primer and translated in the TNT^® T7 Quick Coupled Transcription/Translation System. (1131)

Notes: In this study, Poly(A)+ RNA was isolated from B16 melanoma cell total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for cDNA library construction. The RNAgents^® Total RNA Isolation System was used to isolate total RNA from HEL and K562 human erythroleukemia cells. The isolated RNA was used for RT-PCR. RNasin^® Ribonuclease Inhibitor was used in a very innovative fashion. Peripheral mononuclear cells isolated from blood were directly lysed in a hypotonic buffer containing the inhibitor. The lysates from these cells were used directly for RT-PCR. (2026)