Notes: The authors used the RNAgents® Total RNA Isolation System to isolate RNA from dorsal root ganglia neuron/Schwann cell co-cultures and oligodendrocytes for RT-PCR. (1355)

Notes: Several variant hepatitis B isolates were subjected to PCR to isolate the surface antigen cDNA. The PCR products were cloned and subjected to in vitro transcription/translation with the TNT^® Coupled Reticulocyte Lysate System in the presence of Canine Pancreatic Microsomal Membranes (CMMs). The amount of microsomal membranes was adjusted to give the approximate ratios of wild-type glycosylated and unglycosylated forms. The variants were then tested for immunoprecipitation with a commonly used anti-hepatitis B surface antigen pAb. Only one of the variants was immunoprecipitated. (0520)

Notes: The Access RT-PCR System was used to amplify the lactate dehydrogenase gene from a variety of Antarctic notothenioid fish. (1154)

Notes: The entire coding region of the T isoform of the catalytic subunit of the acetycholinesterase (ACHET)was amplified and cloned into the pTargeT™ Vector and sequenced. The ColQ cDNA was cloned into the pTargeT™ Vector as well and mutated with a Pfu DNA polymerase-based mutagenesis system. The ACHET and ColQ mutants were coexpressed in COS cells and interactions examined with sedimentation analysis through sucrose gradients. (0595)

Notes: Wizard^® PCR Preps DNA Purification System was used to purify PCR products. 100 ng of the PCR products was sequenced using the fmol^® DNA Cycle Sequencing System. (0488)

Notes: Taq DNA polymerase and the  E. coli BL21(DE3) strain were used in these studies. (0263)

Notes: In this paper, the 100bp DNA Ladder was used on 1.5% agarose gel to determine the size of PCR products generated with Taq DNA Polymerase. (2229)

Notes: The Wizard^® Genomic DNA Purification Kit was used to purify genomic DNA from CD45RA+ T cells isolated from cord blood. Cell samples (2-4 X 10^6 cells) were pelleted, washed with PBS, snap frozen in liquid nitrogen and frozen at -70°C prior to isolation. The purified genomic DNA was used for Southern analysis. The DNA was also used for PCR amplification. (0346)

Notes: Cytokine-induced killer (CIK) cells were transfected with an IL-7 expression vector. Cell-mediated cytotoxicity of the CIK cells was tested against A-704 renal carcinoma cells, HT 144 melanoma cells and REH pre B cell leukemic cells using the CytoTox^® 96 Non-Radioactive Cytotoxicity Assay System. In all cases, the transfected CIK cells had greater cytotoxicity to the targets than the mock-transfected or untransfected CIK cells. An MTT reduction assay was used to demonstrate that the transfection was not toxic to the CIK cells. (1740)

Notes: The authors used the CellTiter 96^® Non Radioactive Cell Proliferation Assay to study cell proliferation of IEC-6 and IEC-18 cells (rat intestinal epithelia). (0394)

Notes: The Access RT-PCR System was used to assess IL-8 (interleukin-8) and ENA-78 (epithelial cell-derived neutrophil-activating peptide) mRNA in M. hominis-infected and untreated A549 cells. (0894)

Notes: In this paper, a 538bp fragment was amplified and subcloned into the pGEM^®-T Easy Vector. (1483)

Notes: Total RNA was isolated from rat hepatocytes with the RNAgents^® Total RNA Isolation System. The isolated RNA was treated with DNase and used for RT-PCR. (0416)

Notes: The Transfectam® Reagent was used to transiently transfect NIH 3T3, COS-7 and HeLa cells. A lot of detail is provided for the transfection method. Wizard® Plus Miniprep DNA Purification System and Wizard® PCR Preps DNA Purification System were used for plasmid purification and PCR product purification from low melting point agarose, respectively. (0226)

Notes: RNA probes were produced in the presence of the RNasin^® Ribonuclease Inhibitor. The probes were used in Northern blotting and the sizes of the reactive bands were determined in comparison to the RNA Markers, 0.28-6.58kb. All reagents for RT-PCR (Taq DNA Polymerase; MMLV Reverse Transcriptase; dNTPs and RNasin^® Ribonuclease Inhibitor) were obtained from Promega. (1485)

Notes: The mcp2 sequence was PCR amplified and cloned into the PinPoint^® Xa-1 T Vector. The protein was expressed in JM109 E. coli with IPTG induction. The fusion protein was detected with streptavidin-alkaline phosphatase as well as syphillitic serum preabsorbed with JM109 extracts. (1118)

Notes: Streptavidin MagneSphere^® Paramagnetic Particles (SA-PMPs) were used to capture biotinylated primer probe in a random access retrieval of genetic information by PCR (rargip) screening [ABE, K., (1992) Rapid isolation of desired sequences from lone linker PCR amplified cDNA mixtures: application to identification and recovery of expressed sequences in cloned genomic DNA. Mamm. Genome 2:252-259]. The pGEM^®-T Vector System and PolyATract^® mRNA Isolation System were also used. (0513)

Notes: PCR products were cloned into the PinPoint^® Xa1 T-Vector and expressed in JM109 cells. The cell extracts were electrophoresed and immunoblotted for the specific protein. (0626)

Notes: Total RNA was isolated from ~3 × 10⁶ RAW264.7 macrophages using the SV Total RNA Isolation System. The amount of recovered RNA was adjusted to 500ng/µl and stored at –20°C. One microgram of the RNA was used for RT-PCR analysis. (0066)

Notes: The PolyATtract^® System 1000 was used to isolate polyA+ RNA directly from peripheral blood leukocytes. A 30µl preparation of mRNA was isolated from 1.2 x10⁹ cells. Three microliters of the isolated RNA was used for RT-PCR amplification of the p53 message with the Access RT-PCR System. (1656)

Notes: cDNA synthesis was performed in a reverse transcription reaction containing single-stranded binding protein and RNasin^® Ribonuclease Inhibitor. In a protein truncation test (PTT), five overlapping segments amplified by RT-PCR of NF1 transcript were expressed by TNT^® Coupled Reticulocyte Lysate System. (1051)

Notes: The Access RT-PCR System was used to study expression of three ntn genes from TW3 cells grown in either 4-nitrotoluene, toluene or succinate. The pET-5a Vector (discontinued) was used to express the ntnC protein in E. coli strain BL21(DE3) pLysS. (0968)

Notes: The Access RT-PCR System was used to determine HPRT (hypoxanthine-guanine phosphoribosyltransferase) and iNOS (induced nitric oxide synthase) mRNA in salivary gland lysate of P. papatasi. The Griess Reagent System was used to measure the nitrite accumulation in macrophage culture media. (0191)

Notes: Poly-A+ RNA was isolated from rat cerebellar total RNA with the PolyATtract^® mRNA Isolation System and used for semi-quantitative RT-PCR. The targets of the RT-PCR were cloned with the pGEM^®-T Vector System and sequenced to show they quantitated the correct targets. (0671)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from mouse fibroblasts. The isolated RNA was used for RT-PCR analysis with the Access RT-PCR System. (0474)