Notes: PCR products of 372bp and 426bp were subcloned into the pGEM^®-T Vector and used to produce RNA probes for Northern blotting and in situ hybridization. (1552)

Notes: Superior cervical ganglia were maintained in culture with or without the 2.5S NGF. RNA was isolated from these cultures and used for differential display with RT-PCR. The RT-PCR products were cloned with the pGEM^®-T Vector System. (0998)

Notes: Point mutations in the repeated portion of the PKD1 gene were detected by the Protein Truncation Test. PCR products containing the T7 promoter were used as templates and protein was labeled by incorporation of [³H] leucine in a transcription/translation reaction using TNT^® Coupled Reticulocyte Lysate System. The pGEM^®-T Vector System was also used. (0489)

Notes: PolyA⁺ RNA was isolated from rat total RNA using the PolyATtract^® mRNA Isolation System and used for Northern analysis. The pGEM^®-T Vector System was used to clone products from RT-PCR. (0447)

Notes: Poly(A+) RNA was isolated from total RNA extracted from T47D mammary carcinoma cell line using the PolyATtract^® mRNA Isolation System. The RNA was used for northern blots. RQ1 was used to digest genomic DNA away from total RNA. The pGEM®-T Vector was used to subclone PCR products generated by differential display. (1673)

Notes: The inhibitor was used to protect RNA during RT-PCR. 125I-NGF was produced and used to crosslink to cell surface proteins on PC12 cells. The NGF was also used to assay for neurite outgrowth of PC12 and two clonal variants. Cells were treated with NGF and nuclear extracts made and used to assay for AP1 binding activity. Tyrosine phosphorylation of PI kinase in response to NGF was assayed. Also, the concentration-dependent effect of NGF on the accumulation of 3H-inositol phosphate was examined in the PC12 cells. The bFGF was used to assay the induction of cell differentiation in the PC12 cell and a clonal variant. (1632)

Notes: DNA with unmethylated cytosines converted to uracil by bisulfite treatment was purified with the Wizard^® PCR Preps DNA Purification System. The DNA was used for methylation-specific PCR. (0149)

Notes: The Access RT-PCR System was used in this study. (1998)

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM^®-T Vector and sequenced. (1491)

Notes: Poly A+ RNA was isolated from HeLa cell total RNA using the PolyATtract^® mRNA Isolation System and used for RT-PCR. (1699)

Notes: The RiboMAX™ Large Scale RNA Production System was used to make reference RNA for competitive/quantitative RT-PCR. This is an informative paper about how to perform quantitative RT-PCR and ensure the reaction is linear. The authors developed a series of equations to compensate for nonequality of amplification of different templates. (1461)

Notes: The pCI Mammalian Expression Vector was used to express the p64H1 protein in 293 cells and HT-4, a clonal neuronal cell line. Inserts in the vectors were verified for expression using the TNT^® Coupled Reticulocyte Lysate System. The proteins were translated in the presence of Canine Pancreatic Microsomal Membranes (CMMs). The pGEM^®-T Vector System was used to clone the PCR product of circle rapid amplification of cDNA ends to find the true 5'-end of the p64H1 cDNA. The Tfx™-20 Reagent was used to transfect 293 cells and HT-4 neuronal cells with the p64H1-expressing pCI-based plasmid. (1206)

Notes: The Access RT-PCR System was used to demonstrate expression of the transfected cDNAs in cells. The CellTiter 96^® Non-Radioactive Cell Proliferation Assay (MTT) was used to monitor cell survival after diphtheria toxin exposure. (0698)

Notes: The GDNF E_max ImmunoAssay System was used to quantify the level of GDNF in the conditioned media of HeLa cells transduced with a recombinant adenovirus expressing GDNF. The RQ1 DNase was used to treat RNA sample to remove genomic DNA and the AMV reverse transcriptase was used for first strand cDNA synthesis. (1605)

Notes: The pGEM^®-T Vector System was used to clone a 270bp RT-PCR product from rat spinal cord mRNA.. (2007)

Notes: This article reviews the currently available techniques for screening of DMD and BMD. For BMD, the PTT is not useful for screening for mutation detection because stop mutations are rare in BMD. (1871)

Notes: The authors used Promega's Access RT-PCR System in this study. (2220)

Notes: Tth DNAPolymerase was used to perform a specific first strand cDNA synthesis in a RT-Competitive PCR Assay. (1931)

Notes: The AMV Reverse Transcriptase, RNasin^® Ribonuclease Inhibitor and Taq DNA Polymerase were used for RT-PCR to isolate a clone of the human insulin upstream factor 1, a transcription factor. The protein was expressed in vitro with the Rabbit Reticulocyte Lysate and used for gel shift assays. Reporter studies were performed in MIN6 cells. (0762)

Notes: The Wizard^® Plus Minipreps DNA Purification Resin was used to isolate apoptotic DNA from primary cultures of cerebellar granule cells. The cells were lysed with 7M Guanidine HCl, mixed with 1ml of resin, spun down, resuspended in wash solution, collected in a minicolumn, washed and eluted in water. The DNA was treated with RNase A and treated with the nucleic acid stain, SYBR Green, for quantitation versus a DNA standard. Two hundred nanograms of the isolated DNA were analyzed by agarose gel electrophoresis and ethidium bromide staining. The RQ1 RNase-Free DNase was used to treated total RNA samples prior to RT-PCR. (1217)

Notes: Poly(A+) RNA was isolated from total RNA isolated from various Xenopus tissues. The poly(A+) RNA was used in northern blots and RT-PCR. (1188)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from mouse embryos and oocytes. The isolated RNA was used for RT-PCR analysis. (1659)

Notes: The Dual-Luciferase™ Reporter System was used to quantitate the presenilin promoter activity in Neuro2a neuroblastoma cells, mouse P19 embryonal carcinoma cells and NIH 3T3 cells. Studies were also performed in P19 cells treated with retinoic acid to acquire a neuron-like phenotype and P19 cells treated with dimethyl sulfoxide to acquire a muscle-like phenotype. The presenilin promoter functioned best in the Neuro2a and neuron-like P19 cells. 5´-RACE products from mouse brain RNA were purified with the Wizard^® PCR Preps System and cloned into the pGEM-T Vector . The cloned amplimers were sequenced and used as a template for amplification to produce truncation mutants to assess promoter activity. (1590)

Notes: The Access RT-PCR System was used to amplify both lacZ and bioA in duplex. Thirty-five nanograms of bacterial RNA was amplified for 25 cycles and the amount of lacZ message was normalized to the level of bioA. (0190)

Notes: The PolyATtract^® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM^®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96^® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)