Notes: Taq DNA Polymerase was used in RT-PCR reactions and the products examined by Southern hybridization. Promising bands were cut from the agarose gel and cloned with the pGEM^®-T Vector System. (0529)

Notes: The Access RT-PCR System was used in this study. (1988)

Notes: The authors use the Reverse Transcription System, Wizard^® PCR Preps DNA Purification Systems, Wizard^® Plus Minipreps DNA Purification System and the pGEM^®-T Vector System in their studies. (0445)

Notes: The Access RT-PCR System was used to monitor the expression of the type II iodothyronine deiodinase in primary rat astrocyte cultures following forskolin treatment. (1592)

Notes: PCR products generated by differential display analysis were subcloned into the pGEM^®-T Vector System and subsequently sequenced. (0240)

Notes: The pTargeT™ Vector was used to subclone the 2.5kb Na+/H+ Exchanger 2 and the 2.7kb Na+/H+ Exchanger 3 directly from PCR reactions. The constructs were stably transfected into PS120 Chinese hamster lung fibroblasts and selected for resistance to the neomycin analog, G-418. After 7-10 days of selection, the cells were further tested for expression by RT-PCR and immunoblotting. (1318)

Notes: The Altered Sites^® II in vitro Mutagenesis System was used to introduce four individual amino acid changes into the subtilisin protein. The Wizard^® PCR Preps DNA Purification System was used as well. (0102)

Notes: Glioma cell lines derived from glioblastoma tumors were rapidly screened for premature stop codon or frameshift mutations in the PTEN mRNA by the protein truncation test (PTT). PTT templates were generated by RT-PCR with a T7 RNA polymerase transcription site in the upstream primer and translated in the TNT^® T7 Quick Coupled Transcription/Translation System. (1131)

Notes: In this study, Poly(A)+ RNA was isolated from B16 melanoma cell total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for cDNA library construction. The RNAgents^® Total RNA Isolation System was used to isolate total RNA from HEL and K562 human erythroleukemia cells. The isolated RNA was used for RT-PCR. RNasin^® Ribonuclease Inhibitor was used in a very innovative fashion. Peripheral mononuclear cells isolated from blood were directly lysed in a hypotonic buffer containing the inhibitor. The lysates from these cells were used directly for RT-PCR. (2026)

Notes: The TNT® Coupled Reticulocyte System and Canine Pancreatic Microsomal Membranes (CMMs) were used to express a clone isolated through a differential display screen. (1291)

Notes: The pGEM^®-T Vector System was used to clone the products from PCR differential display. Sequencing of the insert was performed. (1550)

Notes: The MTS-based CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay was used to measure viability of rat glial cells treated with antisense oligodeoxynucleotide. (1539)

Notes: [³⁵S]methionine-labeled pre-IL-1β and PARP(T) proteins were synthesized using the TNT^® T7 Coupled Reticulocyte Lysate System and were used for cleavage studies. The proteins were incubated with purified recombinant human ICE, which cleaved both proteins. The cleavage of PARP requires 50- to 100-fold higher concentrations of ICE than required for pre-IL-1β cleavage. (1060)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from CD4+CD8+ thymocytes. The isolated RNA was used for RT-PCR. (1663)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from CD4+CD8+ thymocytes. The isolated RNA was used for RT-PCR. (0291)

Notes: The PolyATtract^® System 1000 was used to isolate poly A+ RNA directly from the adrenal glands of rats treated with reserpine and from nontreated controls. The isolated poly A+ RNA was used for differential display RT-PCR (1652)

Notes: Total RNA was isolated from the human malaria parasite, Plasmodium falciparum, using the RNAgents^® Assay System. The isolated RNA was used in RT-PCR. (0708)

Notes: RNA was isolated from the human malaria parasite, Plasmodium falciparum using the RNAgents^® Total RNA Isolation System. The isolated RNA was used for RT-PCR. (1660)

Notes: PCR products were cloned into pGEM^®-T Vector System. (0250)

Notes: Poly A+ RNA was isolated from the protozoan Leishmania mexicanna amazonesis total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM-T Vector. (1678)

Notes: PolyATtract^® mRNA Isolation System was used to isolate Poly A+ RNA from the protozoan Leishmania mexicanna amazonesis total RNA. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM^®-T Vector. (0748)

Notes: The inhibitor was used to protect RNA during RT-PCR. (2246)

Notes: PolyA+ RNA was isolated from J2E erythroid cell total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for RT-PCR. (1691)

Notes: The PolyATtract^® System 1000 was used to isolate polyA+ RNA from Pisium sp. plant tissue. Many details of the isolation are given in the paper. The isolated polyA+ RNA was used for RT-PCR and Northern blot analyses. (0736)

Notes: The system was used to isolate poly A+ RNA for INS-1 cell total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used in RT-PCR and Northern analysis. (1700)