Notes: Genomic DNA was isolated from Methanococcus jannaschii using the Wizard^® Genomic DNA Purification Kit. The isolated DNA was used for PCR and eventually expression of the FtsZ protein. (0746)

Notes: The authors used the PolyATtract^® mRNA Isolation System IV to isolate mRNA from total RNA and the Wizard^® PCR Preps DNA Purification Resin to purify a first-strand cDNA synthesis product.  TNT^® T7 Coupled in vitro Transcription/Translation Reticulocyte Lysate System was used to synthesize radiolabeled DNaseY. And to test for signal peptide function they added Canine Pancreatic Microsomal Membranes (CMMs). (0779)

Notes: The authors compared a commercially available enterovirus-specific RT-PCR kit with a kit of their own design. Their 'in-house' RT-PCR system used the Access RT-PCR System to amplify enterovirus sequences from cerebral spinal fluid extracts followed by nested-PCR for final amplification of the target. Both the commercial system and the 'in-house' system were adequate for amplification of enterovirus sequences from CSF of infected individuals. Other, more time-consuming assays were performed to verify the results from the RT-PCR tests. (0545)

Notes: Quantitative RT-PCR was carried out using 1µg total RNA from Arabidopsis using Access RT-PCR System. Samples were taken during the PCR reaction after 5, 10, 15, and 25 cycles and loaded onto agarose gels. Gels were treated for Southern blot hybridization, and filters were hybridized using the AtMYB2 cDNA. Linearity of signal strength was verified using phosphorimaging system quantifications. (1048)

Notes: The Access RT-PCR System was used to amplify two portions of the CHD1 gene product from ostrich poly A+ RNA. The amplification products were gel purified and sequenced. The sequence obtained was compared to that of the human and murine CHD1 and Chicken CHD1Z and CHD1W sequences. (1175)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from normal skin, psoriatic skin, H-128 small cell lung carcinoma cells and peripheral blood mononuclear cells. The isolated RNA was used for RT-PCR performed with the Access RT-PCR System. (0107)

Notes: The fmol^® DNA Cycle Sequencing System was used in this study. (0679)

Notes: Bisulphite-treated DNA was purified using the Wizard® DNA Clean-Up System prior to PCR. The amplified DNA was then cleaned using a Wizard® PCR Preps DNA Purification System prior to cloning and the cloned DNAs were sequenced using the fmol® DNA Cycle Sequencing System. (0001)

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from 3ml S. cerevisiae cultures grown 18-20 hours in YPD broth. The isolated DNA was used for PCR amplification of a 3.5kb segment. The amplimer was purified using the Wizard® PCR Preps System. (0356)

Notes: The authors used the RNAgents® Total RNA Isolation System to isolate RNA from dorsal root ganglia neuron/Schwann cell co-cultures and oligodendrocytes for RT-PCR. (1355)

Notes: Several variant hepatitis B isolates were subjected to PCR to isolate the surface antigen cDNA. The PCR products were cloned and subjected to in vitro transcription/translation with the TNT^® Coupled Reticulocyte Lysate System in the presence of Canine Pancreatic Microsomal Membranes (CMMs). The amount of microsomal membranes was adjusted to give the approximate ratios of wild-type glycosylated and unglycosylated forms. The variants were then tested for immunoprecipitation with a commonly used anti-hepatitis B surface antigen pAb. Only one of the variants was immunoprecipitated. (0520)

Notes: The Access RT-PCR System was used to amplify the lactate dehydrogenase gene from a variety of Antarctic notothenioid fish. (1154)

Notes: The entire coding region of the T isoform of the catalytic subunit of the acetycholinesterase (ACHET)was amplified and cloned into the pTargeT™ Vector and sequenced. The ColQ cDNA was cloned into the pTargeT™ Vector as well and mutated with a Pfu DNA polymerase-based mutagenesis system. The ACHET and ColQ mutants were coexpressed in COS cells and interactions examined with sedimentation analysis through sucrose gradients. (0595)

Notes: Wizard^® PCR Preps DNA Purification System was used to purify PCR products. 100 ng of the PCR products was sequenced using the fmol^® DNA Cycle Sequencing System. (0488)

Notes: Taq DNA polymerase and the  E. coli BL21(DE3) strain were used in these studies. (0263)

Notes: In this paper, the 100bp DNA Ladder was used on 1.5% agarose gel to determine the size of PCR products generated with Taq DNA Polymerase. (2229)

Notes: The Wizard^® Genomic DNA Purification Kit was used to purify genomic DNA from CD45RA+ T cells isolated from cord blood. Cell samples (2-4 X 10^6 cells) were pelleted, washed with PBS, snap frozen in liquid nitrogen and frozen at -70°C prior to isolation. The purified genomic DNA was used for Southern analysis. The DNA was also used for PCR amplification. (0346)

Notes: Cytokine-induced killer (CIK) cells were transfected with an IL-7 expression vector. Cell-mediated cytotoxicity of the CIK cells was tested against A-704 renal carcinoma cells, HT 144 melanoma cells and REH pre B cell leukemic cells using the CytoTox^® 96 Non-Radioactive Cytotoxicity Assay System. In all cases, the transfected CIK cells had greater cytotoxicity to the targets than the mock-transfected or untransfected CIK cells. An MTT reduction assay was used to demonstrate that the transfection was not toxic to the CIK cells. (1740)

Notes: The authors used the CellTiter 96^® Non Radioactive Cell Proliferation Assay to study cell proliferation of IEC-6 and IEC-18 cells (rat intestinal epithelia). (0394)

Notes: The Access RT-PCR System was used to assess IL-8 (interleukin-8) and ENA-78 (epithelial cell-derived neutrophil-activating peptide) mRNA in M. hominis-infected and untreated A549 cells. (0894)

Notes: In this paper, a 538bp fragment was amplified and subcloned into the pGEM^®-T Easy Vector. (1483)

Notes: Total RNA was isolated from rat hepatocytes with the RNAgents^® Total RNA Isolation System. The isolated RNA was treated with DNase and used for RT-PCR. (0416)

Notes: The Transfectam® Reagent was used to transiently transfect NIH 3T3, COS-7 and HeLa cells. A lot of detail is provided for the transfection method. Wizard® Plus Miniprep DNA Purification System and Wizard® PCR Preps DNA Purification System were used for plasmid purification and PCR product purification from low melting point agarose, respectively. (0226)

Notes: RNA probes were produced in the presence of the RNasin^® Ribonuclease Inhibitor. The probes were used in Northern blotting and the sizes of the reactive bands were determined in comparison to the RNA Markers, 0.28-6.58kb. All reagents for RT-PCR (Taq DNA Polymerase; MMLV Reverse Transcriptase; dNTPs and RNasin^® Ribonuclease Inhibitor) were obtained from Promega. (1485)

Notes: The mcp2 sequence was PCR amplified and cloned into the PinPoint^® Xa-1 T Vector. The protein was expressed in JM109 E. coli with IPTG induction. The fusion protein was detected with streptavidin-alkaline phosphatase as well as syphillitic serum preabsorbed with JM109 extracts. (1118)