Notes: The RNAgents^® Total RNA Isolation System was used to isolated total RNA from cultured human gingival fibroblasts. The isolated RNA was used for real-time quantitative PCR. (0648)

Notes: Genomic DNA was isolated from mouse brain with the Wizard^® Genomic DNA Purification Kit. The isolated DNA was used for PCR. The RNasin^® Ribonuclease Inhibitor was used to protect RNA in RT-PCR. (0372)

Notes: In this paper, DNA visualized on a sequencing gel is stained with the SILVER SEQUENCE™ DNA Sequencing Systems, and PCR products are cloned into the pGEM^®-T Vector. (0187)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from immortalized human marrow stromal cell lines, HS-5 and HS-27a, that were incubated with 2 ng/mL IL-1β or 5% conditioned media from HS-5 cell cultures. Poly (A)+ RNA was then isolated from total RNA with the PolyATtract^® mRNA Isolation System. The Poly (A)+ RNA was used in RT-PCR  to create amplimers containing T7 promoters. The PCR products were used as templates in in vitro transcription reactions to generate biotin-labeled probes for microarrays. (2760)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate viral DNA and genomic DNA from Vero cells infected with HSV-1. The isolated DNA was used for PCR. (1393)

Notes: The p53 cDNAs expressed from thymic lymphoma cells were amplified with the Access RT-PCR System and the resulting products were cloned with the pGEM^®-T Vector System. No details of the reaction or whether or not total RNA was used were provided. (0812)

Notes: Synthesis of the mod-D1 cDNA and PCR amplification was performed using the Access RT-PCR System. (0744)

Notes: The complete cDNA for the CD1.1 gene was amplified and subcloned into the pTARGET™ Mammalian Expression Vector. The 1020bp, 339 amino acid protein was stably expressed in CHO cells following selection with G-418 sulfate. Expression was confirmed by Northern blot and FACS analysis with an mAb to the CD1.1 protein. The pGEM®-T Vector System was used for routine cloning of both PCR and RT-PCR products. The Prime-a-gene® Labeling System was used create probes for cosmid library screening. (1303)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from Sertoli cells and germ cells. The isolated RNA was used for RT-PCR-based differential display. (0288)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from a mouse hybridoma. The isolated RNA was used for RT-PCR of the variable regions of both heavy and light chains. The pCI-neo Mammalian Expression Vector was used to express the μ heavy chain in H chain-deficient hybridomas. (1412)

Notes: Total RNA was isolated from Pseudomonas putida using the SV Total RNA Isolation System. RT-PCR using the Access RT-PCR System allowed the authors to characterize the transcriptional organization of several genes involved in naphthalene degradation. (2307)

Notes: The Access RT-PCR System was used to analyze collagen gene expression of primary fibroblasts treated with various agents. The messages were amplified from 100, 200 and 400ng of total RNA. (0928)

Notes: IEC-6 cells were seeded into 96-well plates and cultured in the presence of various concentrations of human IL-17. Cell proliferation was measured using the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay. The pGL3 vector was used to clone a PCR-amplified piece of the 5' flanking region of the CINC gene, and the Luciferase Assay System was used to assess promoter activation. All transfections of IEC-6 cells used the pSV-β-galactosidase vector as a control for transfection efficiency. Total RNA for Northern blotting was isolated using the Poly ATtract System. (2510)

Notes: The pALTER^®-MAX Vector was used to create mutations causing three separate amino acid substitutions on the FKHR cDNA. The Reverse Transcriptase System was used for RT-PCR. (1088)

Notes: The pGEM^®-3Zf(+) Vector was used for routine subcloning and the resulting plasmid was used as a template for PCR. (0114)

Notes: PCR products were purified with the Wizard® PCR Preps DNA Purification System and directly sequenced with the SILVER SEQUENCE™ DNA Sequencing System. (1334)

Notes: Total RNA was isolated from 3-day-old mung bean hypocotyls using the SV Total RNA Isolation System. RT for RT-PCR was performed with the Reverse Transcription System. (0825)

Notes: Total RNA was isolated from 5–10 mouse retinas with the SV Total RNA Isolation System. The isolated RNA was used for Northern analysis and RT-PCR. (1174)

Notes: Total RNA was isolated from mouse eyes using the SV Total RNA Isolation System. Four to six eyes produced sufficient quantities of RNA for Northern analysis (at least three blots at 5µg per lane) and RT-PCR. (0749)

Notes: The SV Total RNA Isolation System was used to extract total RNA from 106 SK-Mel2 human melanoma cells. The isolated RNA was used for RT-PCR with the Access RT-PCR System. The results were used for semi-quantitative analysis. (0959)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from Vibrio harveyi. The isolated DNA was used for PCR. (0060)

Notes: The initial work was performed with a single-tube RT-PCR system from a company in which both the reverse transcriptase and polymerase were mixed together and thus could not be separated. Consequently, the authors could not produce the important no-reverse transcriptase control. To do such a control, the authors used the Access RT-PCR System, which allows such control, since both the Tfl DNA Polymerase and AMV Reverse Transcriptase are added separately to the reaction. (0925)

Notes: The Access RT-PCR System was used for analytical RT-PCR for a variety of targets. Amplification was performed within the linear range of PCR product formation and normalized to the beta-tubulin signal or rpS6 signal. (1169)

Notes: Plasmids were purified with either the Wizard^® Plus Minipreps DNA Purification System or the Wizard^® Plus Midipreps DNA Purifications System. PCR products were purified with the Wizard^® PCR Preps System. (0751)

Notes: Before RT-PCR reactions, the RNA template was treated with RQ1 RNase-free DNase. (0776)