Notes: The RNAgents^® Total RNA Isolation system was used to isolate total RNA from CD4+ and CD8+ T cells isolated from infected and uninfected cattle. The isolated RNA was used for RT-PCR. (0143)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate RNA from LPS-stimulated, bovine leukemia virus-infected peripheral blood mononuclear cells. The RNA was treated with DNase and used for RT-PCR with the Access RT-PCR System. One fiftieth of the reaction was used for nested PCR. (0855)

Notes: The Access RT-PCR System was used to amplify messages for spinophilin and neurabin from rat brain total RNA. The products were used to make probes to screen a cDNA library. (0720)

Notes: The Reverse Transcriptase System was used for two step RT-PCR. (0092)

Notes: Total RNA was isolated from the cortex and cerebellum of adult and developing rat brains with the RNAgents^® Total RNA Isolation System. RT-PCR was performed on a variety of samples with a variety of primers to assay alternative splicing. The Access RT-PCR System was used for all RT-PCR. (0764)

Notes: Taq DNA Polymerase was used extensively to clone the BLINaC cDNA. The resulting clone was put into the pCI Mammalian Expression Vector. Total RNA was isolated from rat liver and primary hepatocytes with the SV Total RNA Isolation System. The RNA was used for RT-PCR. Northern blotting was performed with poly-A(+) RNA isolated with the PolyATtract^® mRNA Isolation System. (0472)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from S. pneumoniae with a slight modification to the standard protocol. The modification was the addition of 0.1% deoxycholate to the lysis solution followed by incubation for 10min. at 37°C. The isolated DNA was used for PCR. (0664)

Notes: The authors used the Access RT-PCR System to perform quantitative RT-PCR from total RNA. (2239)

Notes: The pSP73 Vector was used for routine subcloning. The pGEM®-T Vector System was used for subcloning of PCR products. (1320)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from gingivostomatitis tissue. The RNA was eluted from the basket with 35µl of water, which was passed through the basket three times. Eleven microliters of the eluted material was used for two-step RT-PCR. One microliter of each eluted sample was analyzed for genomic DNA contamination by PCR, and any samples that did amplify were put through the SV RNA System again. As reported by the authors, 'Genomic DNA contamination was undetectable in the vast majority of sample eluates after a single RNA extraction procedure. The remaining samples were subsequently shown to be free from detectable genomic DNA after RNA reextraction.' (1064)

Notes: The ReadyAmp™ Genomic DNA Purification System was used to isolate genomic DNA from 200µl of whole blood. The PCR-quality DNA was used for nested PCR amplification. (0527)

Notes: The factors BDNF and NT-3 were used to maintain cultures. RNasin^® Ribonuclease Inhibitor and Taq DNA Polymerase were used for RT-PCR of rat total RNA derived from arcuate and periventricular cultures from newborn rats as well as intermediate lobe cells from adult rats. (0745)

Notes: Total RNA was extracted from HEL-5J20 and used to generate a full length cDNA (576bp) of the calcium- and intergrin-binding protein using the Access RT-PCR System. The subsequent product was purified with the Wizard^® PCR Preps System, digested with restriction enzymes and cloned into a GST-fusion expression vector. (0214)

Notes: The Apoptosis Detection System, Fluorescein was used to identify apoptotic cells in C6 glioma cultures in response to various reagents. TUNEL Update: The Apoptosis Detection System, Fluorescein has been renamed to DeadEnd™ Fluorimetric TUNEL System. (0504)

Notes: Authors use the PolyATtract^® 1000 System to isolate mRNA from rat myometrium and use this in quantitative RT-PCR experiments. (1220)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from human skin biopsies. Two hundred nanograms of the isolated RNA was used for RT-PCR amplification with the Access RT-PCR System. For added sensitivity, nested PCR was performed as well. (0108)

Notes: The fmol^® DNA Cycle Sequencing System was used to sequence the PCR-amplified G3PDH alleles from a number of haplotypes of cassava. (0604)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate total DNA from virus-infected primary monkey fibroblasts. The isolated DNA was used for PCR. The standard protocol was modified somewhat in that the cells were lysed in 300µl of Nuclei Lysis Solution containing proteinase K overnight at 37°C. The DNA was resuspended at the equivalent of 500, 1000 and 2000 cells per microliter. (0729)

Notes: Subunits of the cDNA for methionine adenosyltransferase were amplified from a plasmid using Taq DNA Polymerase and directly cloned into the pTARGET™ Mammalian Expression T-Vector. The sequence of the cloned inserts were confirmed with the fmol^® DNA Cycle Sequencing System. The constructs were transiently transfected into COS-1 cells with the TransFast™ Reagent at a 1:1 ratio. Excellent detail is provided for the transfection. (1094)

Notes: The PolyATtract® System 1000 was used to isolate poly(A)+ RNA directly from 5637 human bladder carcinoma cells. The isolated RNA was used for RT-PCR. (1272)

Notes: RNA probes were generated with 35S-UTP using the Riboprobe® in vitro Transcription System. The labeled probes were used for in situ hybridization of 18µm sections of the hippocampus. (1298)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from Campylobacter sp. Prior to isolation, the bacteria were washed in TE and resuspended in 1ml of TE at ~1 x 10⁹ cells/ml. The isolated DNA was used for AFLP analysis. (1203)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from RAW 264.7 macrophages treated for various times with endotoxin. The isolated RNA was used for RT-PCR analysis of High Mobility Group-1 gene expression as well as β-actin. RT-PCR was performed with the Access RT-PCR System. (0198)

Notes: Total RNA was isolated from canine left ventricle muscle with the RNAgents^® Total RNA Isolation System. The RNA was used for RT-PCR. T4 Polynucleotide Kinase was used to 5´-end label an oligo to generate a probe for cDNA library screening. (0912)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate total DNA from virus-immortalized T cells. The isolated DNA was used for PCR. (0483)