Notes: The SV Total RNA Isolation System was used to isolate total RNA from freshly isolated human CD4+ T cells. The isolated RNA was used for RT-PCR with the Access RT-PCR System. (2183)

Notes: The PolyATtract^® System 1000 was used to isolated polyA+ RNA directly from IMR90 cells infected with retroviruses expressing the oncogenic ras or an empty vector control. The isolated polyA+ RNA was used as template in RT-PCR reactions containing ³³P-αdCTP. The radiolabeled targets were then hybridized to microarrays (Genome Discoveries) for differential analysis of IMR90 fibroblast gene expression with the ras oncogene expressing retrovirus or an empty retrovirus control. (2695)

Notes: Total RNA was isolated from dorsal root ganglia neurons and used for RT-PCR and differential display PCR. The RNA was isolated using the RNAgents^® Total RNA Isolation System. (2568)

Notes: Various Candida sp. were treated with a zymolase solution then genomic DNA was isolated with the Wizard^® Genomic DNA Purification Kit. The isolated DNA was used for RAPD analysis with Promega's Taq DNA Polymerase, 10X Thermophilic Reaction Buffer and Nuclease-Free Water. Further diagnostic procedures used Promega's dNTP's and Promega's PCR Nucleotide Mix. (0074)

Notes: The SV Total RNA Isolation System was used to isolate RNA from transfected NIH3T3 cells. The isolated RNA was used for RT-PCR. (2159)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from rat kidney medulla and medullary thick ascending limb. The isolated RNA was used in a quantitative RT-PCR. (0073)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from mouse splenocytes, splenic B cells and embryonic fibroblasts. The isolated RNA was used in Northern blots and semi-quantitative RT-PCR. (2567)

Notes: The DeadEnd™ Colorimetric Apoptosis Detection System (DeadEnd™ Colorimetric TUNEL System) was used to analyze apoptotic nuclei in rat gastric sections in response to aspirin. Formalin-fixed, paraffin embedded 40µm sections were analyzed. Cells with nuclei staining dark brown (TUNEL positive) and showing the morphological symptoms of apoptosis were counted to quantitate the number of apoptotic cells. A lot detail is provided about the counting procedure. (1506)

Notes: The Access RT-PCR System was used to assess whether some uncharacterized E. coli sequences that have putative open reading frames are in fact transcribed. The RT-PCR samples contained 0.1µg of bacterial total RNA, and were amplified for 35 cycles. (2158)

Notes: Genomic DNA was extracted from 100 Drosophila melanogaster and 100 Drosophila virilis using the Wizard^® Genomic DNA Purification Kit.  The isolated genomic DNA was used for PCR amplification and direct sequencing.  RT-PCR was also performed in the presence of RNasin^® Ribonuclease Inhibitor and cloned amplimers were purified with the Wizard^® Plus Minipreps DNA Purification System prior to fluorescent sequencing. (2505)

Notes: The SV Total RNA Isolation System was used to isolate RNA from various lung cancer cell lines. The isolated RNA was used for RT-PCR and quantitative RT-PCR. (2161)

Notes: Knockout mice deficient in the tyrosine kinase receptor Kit, which is critical for the development of melanocytes from neural crest-derived precursor cells, were generated. The Kit encoding region was replaced in these mice with the LacZ gene, a convenient marker for Kit expression. Kit-LacZ homozygous mices were identified by PCR using Promega's Taq DNA Polymerase to amplify a 800bp band corresponding to LacZ and to confirm the absence of the 148bp band of Kit. Primary neural crest cell cultures from knockout mouse embryos were immunostained with the Anti-β-Galactosidase mAb to localize Kit expression. Cells were fixed in 4% paraformaldhyde and permeabilized with 0.1% Triton®-X-100 prior to immunostaining. (2422)

Notes: Total RNA was isolated from human serum by combining 100µl of serum with 175µl of SV RNA Lysis Buffer. Only fresh or once-frozen serum was used. The SV Total RNA Isolation System was also used to isolate total RNA from tumor tissue. The rest of the protocol was followed as directed in the technical manual. The quantities of RNA isolated from serum were too low to quantitate so 1µl or 5µl of the isolated RNA was used in RT-PCR to analyze RNA content. (2160)

Notes: The pTARGET™ Mammalian Expression Vector was used to clone and express MIG (monokine-induced by Interferon-gamma) and IP-10 (interferon-inducible protein 10) in A549 human adenocarcinoma cells. Stable transfectants were obtained through G418 selection. (2058)

Notes: Poly(A)⁺ RNA was purified from total RNA by the PolyATtract^® mRNA Isolation System. PCR products were purified by agarose gel electrophoresis and were cloned into pGEM^®-T and pGEM^®-T Easy Vector. Genes were obtained by RT-PCR using the Access RT-PCR System. (1097)

Notes: PCR products were used in TNT^® T7 Coupled Reticulocyte Lysate System. (1215)

Notes: Total RNA was isolated from a virulent strain of Salmonella enterica, SL1344 using the SV Total RNA Isolation System. The Access RT-PCR System was used to characterize the transcriptional organization of the prg operon. The pgrH gene was amplified by RT-PCR and cloned into the pGEM^®-T vector (2305)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from various rat tissues. The isolated RNA was used for RT-PCR. The authors also use a derivative of the pCI Vector called pCI-IRES-CD8 to express the TWIK protein specifically in COS cells. (2179)

Notes: Poly A+ RNA was isolated from infected cynomolgus monkey plasma and used for RT-PCR with the Access RT-PCR System. (0396)

Notes: Total RNA was extracted from bovine herpesvirus 1-infected and -uninfected MDCK canine kidney, and CV-1 monkey kidney cell lines using the RNAgents^® Total RNA Isolation System. The isolated RNA was used for RT-PCR. (1227)

Notes: The Access RT-PCR System was used to amplify Vα₁₀Jα₂₄ mRNA of the TCR alpha chain and the resulting product was cloned with the pGEM^®-T Vector System. (0734)

Notes: Total RNA was isolated from uninfected Vero cells and Vero cells infected with either Simkania negevensis Z^T or Chlamydia trachmatis using the SV Total RNA Isolation System. The isolated RNA was used in RT-PCR amplifications to determine the size of the unspliced intron form of the 23S rRNA to differentiate between a group I intron where the rRNA intron is spliced with religation and an intervening segment where the rRNA is fragmented to functionally remove the intervening segments. The isolated RNAs and Promega's RNA Markers were separated by electrophoresis to judge RNA integrity. (2302)

Notes: The authors used the Tfx™-50 Reagent to transfect bovine aortic endothelial cells. They also used the pGEM^®-T Easy Vector System to clone PCR products. (1482)

Notes: Authors use the Wizard^® Genomic DNA Purification Kit to isolate DNA from newborn mice livers. They performed nested PCR on V(H)-D-J(H) genes and cloned these into the pGEM^®-T Vector and sequenced the rearrangements using the fmol^® DNA Cycle Sequencing System. (0908)

Notes: The SV Total RNA Isolation System was used to isolate RNA from human peritoneal mesothelial cells. The isolated RNA was used for RT-PCR. (2164)