Notes: The Wizard^® Lambda DNA Purification System was used to isolate phage for the Sphingomonas paucimobilis and Flavobacterium sp. isolates. The isolated DNA was used for determination of the phage genome size by restriction digestion. The Wizard^® Genomic DNA Purification Kit was used to isolate the genomic DNA from these bacterial species as well. The authors note that the DNA isolated with the Wizard^® Genomic Kit was as pure as that purified by a cesium chloride gradient. Loading of the DNA isolated with the Genomic Kit onto cesium chloride gradients produced only a single sharp band after centrifugation. The isolated DNA was used for dot blot hybridizations. (0979)

Notes: In this paper, Tfl DNA polymerase was used for two-step RT-PCR. The amplified fragments were cloned into the pGEM®-T Vector. Promega's Terminal Deoxynucleotidyl Transferase (TdT) was used to end label specific oligos for screening a frog brain cDNA library. T7 and T3 RNA polymerases were used to make digoxigenin labeled riboprobes for in situ hybridization. (1281)

Notes: The pGEM®-T Easy Vector was used to clone PCR products amplified from the prophage inserts of various lysogenic Lactococcus strains. (0193)

Notes: The ProFection® Mammalian Transfection System - Calcium Phosphate was used to transfect HeLa and HeLa-Tat cell lines. The authors developed a vector-antiviral gene system for use in gene therapy in the treatment of HIV-1. pGEM®-luc was used as a negative control in luciferase assays of transfected cells. (1388)

Notes: In this paper, a 538bp fragment was amplified and subcloned into the pGEM^®-T Easy Vector. (1483)

Notes: Poly(A)+ RNA was purified with PolyATtract^® mRNA Isolation Systems. Anti-Rabbit IgG AP Conjugate, and BCIP/NBT were used in Western blotting. R transcripts were synthesized from the Lc cDNA in the pGEM-7Z(+) Vector, and were used in in vitro translation by Rabbit Reticulocyte Lysate Systems to be used as a positive control in Western. (0781)

Notes: These authors used Promega's pGEM^®-T Easy Vector Systems, Taq DNA Polymerase and Tli DNA Polymerase in this paper. (2028)

Notes: Streptavidin MagneSphere^® Paramagnetic Particles (SA-PMPs) were used to capture biotinylated primer probe in a random access retrieval of genetic information by PCR (rargip) screening [ABE, K., (1992) Rapid isolation of desired sequences from lone linker PCR amplified cDNA mixtures: application to identification and recovery of expressed sequences in cloned genomic DNA. Mamm. Genome 2:252-259]. The pGEM^®-T Vector System and PolyATract^® mRNA Isolation System were also used. (0513)

Notes: Both the pGEM^®-5Zf(+) Vector and the pGEM^®-7Zf(+) Vector were used for routine subcloning of restriction fragments of the PEX20 gene in preparation for sequencing. (0269)

Notes: Poly-A+ RNA was isolated from rat cerebellar total RNA with the PolyATtract^® mRNA Isolation System and used for semi-quantitative RT-PCR. The targets of the RT-PCR were cloned with the pGEM^®-T Vector System and sequenced to show they quantitated the correct targets. (0671)

Notes: ()

Notes: The pGEM®-5Zf(+) Vector was used for subcloning of a PCR fragment. The fragment was used to make a probe for RNase protection assays. The pGEM®-7Zf(+) Vector was used for routine subcloning of restriction fragments from the chitotriosidase gene. (1427)

Notes: Taq DNA Polymerase was used for PCR. The PCR products were purified with the Wizard^® PCR Preps DNA Purification System and cloned with the aid of the pGEM^®-T Vector System. (0624)

Notes: The pGEM^®-13Zf(+) Vector was used for subcloning. (0986)

Notes: RT-PCR was performed with degenerate primers and the resulting 110bp product was subcloned with the pGEM®-T Vector System. The 110bp fragment was used to screen a cDNA library and four positive plaques were identified. (0650)

Notes: Various portions of a 552 residue protein were cloned and expressed in BL21(DE3)pLysS using the pGEMEX®-1Vector. The resulting proteins were purified and used as immunogens. The TNT® Coupled Reticulocyte Lysate System was used for in vitro translation. (1236)

Notes: The RNasin^® Ribonuclease Inhibitor and the pGEM^®-T Vector System were used in this study. The inhibitor was used to protect RNA during the T4 RNA ligase step for 5' RACE analysis. (1643)

Notes: Promega's pGEM®-T Vector System, pCI Mammalian Expression Vector and TNT® Coupled Reticulocyte Lysate System were used in this study. (1951)

Notes: A portion of the cytochrome oxidase subunit I cDNA was amplified and cloned into the pGEM^®-T Vector. RNA probes were produced from the pGEM^®-T Vector for in situ hybridization studies. (1547)

Notes: The ProFection^® Mammalian Transfection System-CaPO₄ was used to transiently transfect 293T cells. (0521)

Notes: NGF was used to induce PC12 cell differentiation 3-5 days prior to assay. PCR amplimers representing various subunits of the acetylcholine receptor were subcloned into the pGEM^®-T Vector. The resulting clones were used to produce RNA probes for RNase protection assays. Also, a chimeric a5/7-HT3 receptor was generated by PCR, subcloned into the pGEM^®-T Vector and sequenced. (1419)

Notes: The pGEM^®-T Vector System was used in this study. (2009)

Notes: The Altered Sites^® II in vitro Mutagenesis System was used to produce several vitamin D receptor truncation mutants. The mutants were generated by the insertion of premature stop codons. The single-stranded protocol was used for the synthesis reaction and the newly synthesized DNA was propagated in BMH 71-18 mutS cells followed by growth in JM109 cells. Both mutant and wildtype receptor were cloned into pGEM^®-4 Vector and in vitro expressed with the TNT^® Coupled Reticulocyte Lysate System. The various forms of the receptor were assessed for protease sensitivity in the presence or absence of ligand. (0783)

Notes: Promega's pGEM®-T Vector System and TNT® Coupled Reticulocyte Lysate System were used in this study. (1948)

Notes: Taq DNA Polymerase was used in RT-PCR reactions and the products examined by Southern hybridization. Promising bands were cut from the agarose gel and cloned with the pGEM^®-T Vector System. (0529)