Notes: The function of latency-associated nuclear antigen (LANA) in latent DNA replication of the Kaposi’s sarcoma-associated herpesvirus (KSHV) is investigated. To determine the localization of LANA, the HaloCHIP™ System was used. Interestingly, localization of the DNA-binding domain of LANA to the nuclear matrix helped in facilitating viral replication. Fusion constructs to nuclear mitotic apparatus protein (NuMA) showed significantly stronger ori-P binding and replication. (5096)

Notes: The function of the regulatory element, 5.5URR, upstream of signal transducer and activator of transcription 1 (STAT1) is investigated. The Dual-Luciferase® Reporter Assay showed a significant increase in transcription in the presence of the 5.5URR upon interferon treatment. The HaloCHIP™ System showed a physical interaction between the 5.5URR element and the STAT1 promoter, which was stimulated by interferon treatment. Together, the 5.5URR element may serve in maintaining interferon signaling in relation to STAT1. (5097)

Notes: RNA was extracted from cultured A549 cells and used in RT-qPCR to analyze the effect of transfected siRNAs. (4444)

Notes: To study the role of the transcription factor Sox9 in the transition from cartilage to bone in newborn mice, the authors performed chromatin immunoprecipitation using the HaloCHIP™ System. Primary rib chondrocytes were transfected with a vector expressing full-length Sox9 with a HaloTag^® protein tag, then proteins and DNA were cross-linked. DNA was isolated and sonicated, and the Sox9:DNA complexes were precipitated using the HaloLink™ Resin. The precipitated DNA then was amplified by PCR to determine that SOX9 binds to the SRY sites in the Vegfa gene. (4054)

Notes: The HaloCHIP System was used to measure CREB binding as an alternative to the ChIp method. (4057)

Notes: The authors used a HaloTag^® vector and the HaloCHIP™ System to identify a KLF15 binding site in the HSD17B5 promoter. (4059)