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Virology 500, 190–7. A chimeric protein composed of NuMA fused to the DNA binding domain of LANA is sufficient for the ori-P-dependent DNA replication. 2017

Ohsaki, E. and Ueda, K.

Notes: The function of latency-associated nuclear antigen (LANA) in latent DNA replication of the Kaposi’s sarcoma-associated herpesvirus (KSHV) is investigated. To determine the localization of LANA, the HaloCHIP™ System was used. Interestingly, localization of the DNA-binding domain of LANA to the nuclear matrix helped in facilitating viral replication. Fusion constructs to nuclear mitotic apparatus protein (NuMA) showed significantly stronger ori-P binding and replication. (5096)

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Genes Cells 21(1), 25–40. Distal regulatory element of the STAT1 gene potentially mediates positive feedback control of STAT1 expression. 2016

Yuasa, K. and Hijikata, T.

Notes: The function of the regulatory element, 5.5URR, upstream of signal transducer and activator of transcription 1 (STAT1) is investigated. The Dual-Luciferase® Reporter Assay showed a significant increase in transcription in the presence of the 5.5URR upon interferon treatment. The HaloCHIP™ System showed a physical interaction between the 5.5URR element and the STAT1 promoter, which was stimulated by interferon treatment. Together, the 5.5URR element may serve in maintaining interferon signaling in relation to STAT1. (5097)

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Nucl. Acids Res. 41, 8515–25. Pin1 promotes GR transactivation by enhancing recruitment to target genes. 2013

Poolman, T.M., Farrow, S.N., Matthews, L., Loudon, A.S. and Ray, D.W.

Notes: RNA was extracted from cultured A549 cells and used in RT-qPCR to analyze the effect of transfected siRNAs. (4444)

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Development 137, 901–11. SOX9 is a major negative regulator of cartilage vascularization, bone marrow formation and endochondral ossification. 2010

Hattori, T., Müller, C., Gebhard, S., Bauer, E., Pausch, F., Schlund, B., Bösl, M.R., Hess, A., Surmann-Schmitt, C., von der Mark, H., de Crombrugghe, B. and von der Mark, K.

Notes: To study the role of the transcription factor Sox9 in the transition from cartilage to bone in newborn mice, the authors performed chromatin immunoprecipitation using the HaloCHIP™ System. Primary rib chondrocytes were transfected with a vector expressing full-length Sox9 with a HaloTag® protein tag, then proteins and DNA were cross-linked. DNA was isolated and sonicated, and the Sox9:DNA complexes were precipitated using the HaloLink™ Resin. The precipitated DNA then was amplified by PCR to determine that SOX9 binds to the SRY sites in the Vegfa gene. (4054)

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BMC Genomics 10, 497. A functional analysis of the CREB signaling pathway using 2009

Danette D Hartzell, Nathan D Trinklein, Jacqui Mendez, Nancy Murphy, Shelley F Aldred, Keith Wood and Marjeta Urh

Notes: The HaloCHIP System was used to measure CREB binding as an alternative to the ChIp method. (4057)

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J. Clin. Endocrinol. Metab. 94, 2594-2601. KLF15 Is a transcriptional regulator of the human 17beta-hydroxysteroid dehydrogenase type 5 gene. A potential link between regulation of testosterone production and fat stores in women 2009

Du, X., Rosenfield, R. and Qin, K.

Notes: The authors used a HaloTag® vector and the HaloCHIP™ System to identify a KLF15 binding site in the HSD17B5 promoter. (4059)

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