Notes: Fragments of the pp65 protein cDNA were subcloned into a modified pCI-neo Mammalian Expression Vector containing an enterokinase cleavage site and 6XHis tag. These fusion vectors were co-transfected into COS cells with an HLA A*0201 gene-expressing pCI-Neo Vector construct. The transfected cells were assayed for TNFα production. TNFalpha was quantified by its cytotoxicity to WEHI-164 cells using the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS). (1231)

Notes: Several proteins were transiently expressed in the 1471.1 cell line using the pCI Mammalian Expression Vector. (0379)

Notes: The pCI-neo Mammalian Expression Vector was used in a rather unique way. The neo gene was replaced with a transferrin receptor cDNA and another protein was subcloned downstream of the CMV promoter. Both proteins were expressed in CHO cells. Stable transfectants were obtained by cotransfection with the vector pZeo (Invitrogen) expressing yet a third protein. (0164)

Notes: All three isoforms of JNK were subcloned into the pCI-neo Mammalian Expression Vector and a hemagglutinin tag. Expression vectors were also constructed with HPK1 and MEKK1 using the pCI-neo Mammalian Expression Vector. Constructs were expressed in 293T cells. Extracts of cells expressing either of the constructs were assayed for the ability to phosphorylate a p53-GST fusion protein. Expressed proteins were assayed for p53 interactions by co-expression followed by immunoprecipitation (1020)

Notes: The mevalonate transporter protein gene was cloned into the  pCI-neo Mammalian Expression Vector and stably transfected into the DLD-1 cell line. Two mutant K-Ras protein genes and one mutant N-ras protein gene were cloned into the  pCI-neo Mammalian Expression Vector and transiently expressed in NIH3T3 cells and COS-7 cells. (0183)

Notes: The pCI-Neo Mammalian Expression Vector was used in conjunction with a luciferase reporter vector to determine the important regions on the carboxy-terminal portion of the 386-amino acid LMP-1 protein. Both full-length and truncation mutants were expressed in the BJAB human EBV-negative lymphoblastoid B cell line. Luciferase activity was measured using the Luciferase Assay System. (1409)

Notes: The pCI-neo Mammalian Expression Vector was used to express a 554-amino acid hyaluronan synthase in COS-1 cells. (0363)

Notes: A fusion protein of the extracellular domain of the tyrosine kinase receptor Nsk2 was fused to the Fc portion of mouse immunoglobulin and subcloned into the pCI-neo Mammalian Expression Vector. The construct was stably expressed from 293 cells. The protein was recovered from the conditioned media by Protein A affinity chromatography. The protein was used to create an anti-Nsk2 antisera. (0676)

Notes: The pCI Mammalian Expression Vector was used to express a flag-tagged 497 amino acid potassium channel in COS cells. (0433)

Notes: The pCI-neo Mammalian Expression Vector was used to transiently express the 282 amino acid p32 protein and a 73 N-terminal amino acid truncation in PCL cells. Cells were assayed by Western blotting and immunocytochemistry. Transfections were performed with the Transfectam^® Reagent. (0636)

Notes: The 676 amino acid KvLQT1 protein and site-directed mutants were cloned into the pCI Mammalian Expression Vector, expressed in COS cells and assayed for activity. (1297)

Notes: The pCI-neo Mammalian Expression Vector was used to express three proteins, bcl-2, bcl-2(G145A), and Bax in 293 cells. Expressed proteins were used for in vivo apoptosis studies. (0572)

Notes: The pCI-neo Mammalian Expression Vector was used to express the 1400bp EWS/FLI-1 protein in fibroblast W- and R- cells which are cells derived from transgenic mice either expressing the wildtype IGF-1 receptor (W) or a knockout of the receptor (R). (0278)

Notes: Both pCI and pCI-neo Mammalian Expression Vectors were used to express proteins in Mouse LTA and NIH3T3 cells and human HeLa cells. The authors report, 'In general we observed that even after optimization of the transfection conditions the expression level of the pCI-neo constructs was only 25% compared with pCI constructs.' (1122)

Notes: Both ZEB and the DNA-binding domain of ZEB were subcloned into Promega's pCI-neo Mammalian Expression Vector just behind a FLAG epitope. After cotransfection with reporter vectors, the effect of the expressed protein was assessed in 10T1/2 fibroblasts and C2C12 cells. The two protein inserts were subcloned into a T3 RNA polymerase binding site bearing plasmid and the flag-tagged proteins were expressed in vitro with the TNT^® Coupled Reticulocyte Lysate System. (0542)

Notes: The pCI-neo Mammalian Expression Vector was used to produce stable CHO cell transfectants expressing a fusion of selectin fused to the hinge and Fc regions of human IgG1 heavy chain. (0594)