Citations Search

Sort By:

Brain 141, 2329–42. Impaired plasticity of  macrophages in X-linked adrenoleukodystrophy. 2018

Weinhofer, I., Zierfuss, B., Hametner, S., Wagner, M., Popitsch, N., Machacek, C., Bartolini, B., Zlabinger, G., Ohradanova-Repic, A., Stockinger, H., Köhler, W., Höftberger, R., Regelsberger, G., Forss-Petter, S., Lassmann, H. and Berger, J.

Notes: The authors use whole transcriptome analysis to investigate monocyte expression profiles in X-linked adrenoleukodystrophy. Viability and glutathione oxidative balance of monocytes was measured using the MultiTox-Glo and GSH/GSSG-Glo™ assays on the GloMax® system. (5178)

Expand Full Notes »

Current Chemical Genomics 3, 33-41. In vitro viability and cytotoxicity testing and same-well multi-parametric combinations for high-throughput screening 2009

Niles, A.L., Moravec, R.A. and Riss, T.L.

Notes: The authors review the use of in vitro cytotoxicity testing in drug discovery to characterize the toxic potential of new chemical entities (nce) at the earliest stages of profiling. DOI: 10.2174/1875397300903010033 (4000)

Expand Full Notes »

Expert Opin. Drug Metab. Toxicol. 4, 103–120. Bioluminescent assays for ADMET 2008

Cali, J.J., Niles, A., Valley, M.P., O’Brien, M.A., Riss, T.L., and Shultz, J.

Notes: The authors of this review article highlight the use of bioluminescence as a readout for high-throughput ADME/Tox assays. They discuss three strategies for designing bioluminescent assays, using either luciferase, ATP or luciferin substrates as the limiting reagents for a luciferase-catalyzed reaction. Reporter gene assays limit the production of luciferase by tying it to a promoter or DNA regulatory region of interest. Such assays can be used to study genes that are regulated by drugs and other xenobiotics. Bioluminescent assays in which ATP is the limiting reagent of the luciferase reaction can be designed to monitor cell viability or the activity kinases. Bioluminescent assays in which the substrate is limiting can be designed so that the activity of a particular enzyme results in the production of a luciferin substrate that can, in turn, be acted upon by luciferase. (3926)

Expand Full Notes »

Anal. Biochem. 366, 197–206. A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers 2007

Niles, A.L., Moravec, R.A., Hesselberth, P.E., Scurria, M.A., Daily, W.J. and Riss, T.L.

Notes: The authors of this paper describe an assay that uses protease biomarkers to assess cell viability and cell death simultaneously in a population of cells. The assay detects an ubiquitous protease activity that is associated with live cells and a second protease activity that is associated with cells that have lost membrane integrity. The readouts are either fluorescent or fluorescent and luminescent. The assay can be performed in multiplex with other assays, such as caspase assays, to gain additional information on the cell population, and it is amenable to high-throughput screening. (3927)

Expand Full Notes »