Notes: In this paper, biotinylated and fluorescently labeled DNA probes were used simultaneously in hybridization reactions with restriction digested human genomic DNA. Streptavidin-paramagnetic particles were used to capture restriction fragments of interest prior to analysis for CpG methylation and short-nucleotide repeats with an alkaline-phosphatase-conjugated anti-fluorescein antibody.  These immuno-DNA complexes were then analyzed using the AttoPhos^® AP Fluorescent Substrate System in 96-well plates. Fluorescence signal was determined using a FluoroCount™ microplate reader. Details of  hybridization conditions, probe concentrations, washes and statistical analysis are provided. (2655)

Notes: These researchers created alkaline phosphatase-conjugated probes that were specific for the α-proteobacterium Rhodospirillum rubrum RubisCO gene, rbcL. These probes were used in Southern and Northern blot analysis of non-transformed and transformed tobacco rubrum plants using the AttoPhos® AP Fluorescent Substrate System, a Vistra Fluorimager™ and ImageQuant™ software. The AttoPhos® Substrate was also used to visualize immunoblots of various proteins in transformed and non-transformed plants. To confirm their results, the researchers PCR-amplified choroplast DNA and ligated the products into the pGEM®-T Easy Vector for BigDye® Sequencing. (2649)