Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Citations Search

Sort By:

Sci. Rep. 6, 28231. Fast and high resolution single-cell BRET imaging. 2016

Goyet, E., Bouquier, N., Ollendorff, V. and Perroy, J.

Notes: Nanoluciferase was used to characterize the conditions required to reliably perform single-cell BRET imaging of protein-protein interaction. HEK293T cells were cultured and transfected with vectors containing the Nluc gene sequence, such as is provided in the pNL1.1 Vector. Extracellular signal-related kinase (ERK) activity in neuronal dendritic spines, induced by the activation of endogenous synaptic NMDA receptors, was detected by an Nluc-optimized BRET-based sensor of ERK activity. The authors report that the use of Nanoluciferase improved the resolution in terms of both time and space, enhanced the duration of signal stability, expanded the dynamic range and increased the sensitivity of the BRET signals in single-cell imaging. (4691)

Expand Full Notes »

Cell 97(1), 29-39. A Smad transcriptional corepressor. 1999

Wotton, D., Lo, R.S., Lee, S. and Massague, J.

Notes: The pRL-CMV plasmid was used as an internal transfection efficiency control. The authors used a homebrew Renilla assay system. The buffer used was 25mM Tris (pH 7.5), 100mM NaCl, with 0.09µM coelenterazine (Biosynth) from an 0.09µM stock in 20mM HCl in methanol. (2170)

Expand Full Notes »