Notes: The authors of this study used next generation sequencing technology to assemble chloroplast genome and detect single nucleotide polymorphisms for a rainforest tree species. Genomic DNA was extracted from leaf samples, and 2-3 extractions per individual tree were pooled. Genomic DNA was quantitated using the QuantiFluor® dsDNA System on the SpectraMAX Gemini XPS detector prior to next generation sequencing. (4509)

Notes: The authors of this study sought to apply a genotyping-by-sequencing approach to genotype cattle from the US and Africa. They collected blood samples from 47 unrelated animals slaughtered in the US and Nigeria. Genomic DNA was extracted and then quantitated using the QuantiFluor® dsDNA System on a SpectraFluor Plus plate-format fluorometer before being used for next-generation sequencing. (4508)

Notes: The authors extracted DNA from dormant vegetative buds and quantitated DNA using the QuantiFluor® dsDNA System on a SpectraFluor Plus plate-format fluorometer prior to NGS (4917)

Notes: In this study, the authors evaluated “genotyping-by-sequencing” for the economically important lodgepole pine and white spruce conifer species. DNA samples extracted from dormant vegetative buds and DNA was quantitated using Quantifluor® ds DNA System on a SpectraFluor Plus plate-format fluorometer. (4507)

Notes: The authors performed ChIP and quantitated immunoprecipitated DNA with the QuantiFluor® dsDNA System prior to NGS on an Illumina Instrument. (4918)

Notes: The authors studied the effect of butyrate treatment on histone acetylation and characterized how H3 acetylation affects DNA sequence binding specificity. To examine sequence specificity, they performed chromatin immunoprecipitation with butyrate-treated and untreated bovine kidney epithelial cells, quantified the recovered DNA, then sequenced the DNA by Illumina next-generation sequencing. DNA was quantitifed using the QuantiFluor® dsDNA System. (4240)