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Citations Search

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Eur. J. Nutr. 53, 1051–1064. Association of dietary type with fecal microbiota in vegetarians and omnivores in Slovenia 2014

Bogovič, B. M., Obermjer, T., Lipoglavšek, L, Grabnar, I., Avguštin, G. and Rogelj, I.

Notes: The authors of this study used real time-qPCR and PCR-DGGE (denaturing gradient gel electrophoresis) to analyze and compare the mixed bacterial systems from fecal samples of vegetarians and omnivores. Bacterial DNA was isolated from frozen fecal samples using the Maxwell® 16 Tissue DNA Purification Kit, and PCR-DGGE reactions were set up using GoTaq® Flexi DNA Polymerase and GoTaq® Flexi Colorless Reaction Buffer. The authors of this study were able to detect differences in microbiota that seemed to be related to diet. (4523)

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Folia Microbiol. 58, 623–30. Detection and quantification of probiotic strain Lactobacillus gasseri K7 in faecal samples by targeting bacteriocin genes. 2013

Treven, P., Turkova, K., Trmčić, A., Obermajer, T., Rogelj, I. and Matijašić, B.B.

Notes: The authors were interested in quantitating the presence as well as the prevalence of Lactobacillus gasseri K7 in humans that did not consume the probiotic bacteria. Fecal samples from 45 healthy adults were collected, frozen, diluted, centrifuged and digested with proteases. After sonication, DNA was extracted using the Maxwell® 16 Tissue DNA Purification kit on the Maxwell® 16 instrument. This same kit and instrument also were used to isolate bacterial DNA from 1ml bacterial cultures. The purified DNA was PCR amplified using primers for gassericin K7 A and K7 B (bacteriocin) genes and GoTaq® Flexi DNA Polymerase in Green GoTaq® Flexi Buffer for 30 cycles. PCR products were analyzed by 1.8 % agarose gel electrophoresis. (4522)

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Nucl. Acids Res. 39, e81. A method for counting PCR template molecules with application to next-generation sequencing.
2011


J.A. Casbon, R. J. Osborne, S. Brenner and C.P. Lichtenstein

Notes: Human Genomic DNA used as the starting material in the NGS workflow.  GoTaq® Flexi Colorless Buffer and GoTaq® Flexi Polymerase were used in amplification of template in step added to the beginning of library preparation. (4531)

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