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Biotechniques 51, 105–110. A bioluminescent assay for the sensitive detection of proteases. 2011

Leippe, D.M., Nguygen, D., Zhou, M., Good, T., Kirkland, T., Scurria, M., Bernad, L., Ugo, T., Vidugiriene, J., Cali, J.J., Klaubert, D. and O'Brien, M.

Notes: The ability to detect trace protease activity is important for assessing protein purification methods during process development and for confirming the absence of protease in final purified proteins. The authors describe a general protease assay using five peptide-conjugated luciferase substrates and compare it to fluorophore-conjugated general protease assays. (4156)

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