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Mol. Cell. Biol. 23, 7875–7886. Molecular mechanism for a role of SHP2 in epidermal growth factor receptor signaling. 2003

Agazie, Y.M. and Hayman, M.J.

Notes: Researchers used the AttoPhos® AP Fluorescent Substrate System to visualize immunoblot bands. Band intensities were  quantified by phosphoimager analysis.  The relative intensities of the bands containing phosphorylated proteins were compared to those of unstimulated samples. (2793)

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EMBO J. 21, 6358-6366. BSE prions propagate as either variant CJD-like or sporadic CJD-like prion strains in transgenic mice expressing human prion protein. 2002

Asante, E.A., Linehan, J.M., Desbruslais, M., Joiner, S., Gowland, I., Wood, A.L., Welch, J., Hill, A.F., Lloyd, S.E., Wadsworth, J.D.F. and Collinge, J.

Notes: AttoPhos® AP Fluorescent Substrate System was used to quantify and analyze differential molecular weight bands of glycosylated prion strains taken from various of wildtype and transgenic mice. The researchers used a Storm® 840 PhosphorImager with ImageQuant™ software to visualize and quantify the bands. Scatterplots were used to display the  populations of prion isoforms. (2650)

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Clin. Chem. 48, 1844-1850. High-throughput detection of submicroscopic deletions and methylation status at 15q11-q13 by a photo-cross-linking oligonucleotide hybridization assay. 2002

Peoples, R., Weltman, H., Van Atta, R., Wang, J., Wood, M., Ferrante-Raimondi, M., Cheng, P. and Huan, B.

Notes: In this paper, biotinylated and fluorescently labeled DNA probes were used simultaneously in hybridization reactions with restriction digested human genomic DNA. Streptavidin-paramagnetic particles were used to capture restriction fragments of interest prior to analysis for CpG methylation and short-nucleotide repeats with an alkaline-phosphatase-conjugated anti-fluorescein antibody.  These immuno-DNA complexes were then analyzed using the AttoPhos® AP Fluorescent Substrate System in 96-well plates. Fluorescence signal was determined using a FluoroCount™ microplate reader. Details of  hybridization conditions, probe concentrations, washes and statistical analysis are provided. (2655)

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Proc. Natl. Acad. Sci. USA 98, 14738-14743. Plastome-encoded bacterial ribulose-1,5-bisphosphate carboxylase oxygenase (RubisCO) supports photosynthesis and growth in tobacco. 2001

Whitney, S.M. and Andrews, J.T.

Notes: These researchers created alkaline phosphatase-conjugated probes that were specific for the α-proteobacterium Rhodospirillum rubrum RubisCO gene, rbcL. These probes were used in Southern and Northern blot analysis of non-transformed and transformed tobacco rubrum plants using the AttoPhos® AP Fluorescent Substrate System, a Vistra Fluorimager™ and ImageQuant™ software. The AttoPhos® Substrate was also used to visualize immunoblots of various proteins in transformed and non-transformed plants. To confirm their results, the researchers PCR-amplified choroplast DNA and ligated the products into the pGEM®-T Easy Vector for BigDye® Sequencing. (2649)

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