Peoples, R., Weltman, H., Van Atta, R., Wang, J., Wood, M., Ferrante-Raimondi, M., Cheng, P. and Huan, B.
Notes: In this paper, biotinylated and fluorescently labeled DNA probes were used simultaneously in hybridization reactions with restriction digested human genomic DNA. Streptavidin-paramagnetic particles were used to capture restriction fragments of interest prior to analysis for CpG methylation and short-nucleotide repeats with an alkaline-phosphatase-conjugated anti-fluorescein antibody. These immuno-DNA complexes were then analyzed using the AttoPhos® AP Fluorescent Substrate System in 96-well plates. Fluorescence signal was determined using a FluoroCount™ microplate reader. Details of hybridization conditions, probe concentrations, washes and statistical analysis are provided. (2655)