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Proc. Natl. Acad. Sci. USA 94, 2204-2209. A putative angiogenin receptor in angiogenin-responsive human endothelial cells. 1997

Hu, G.F., Riordan, J.F., Vallee, B.L.

Notes: The authors used rhFGF, Basic, and anti-acidic FGF antibody (antibody has been discontinued) to analyze the effect of these factors on the activity of angiogenin on endothelial cells. They also labeled surface molecules of endothelial cells with biotin following treatment with angiogenin to aid in purification of angiogenin receptor. Then they took biotin-labeled molecules and purified using SoftLink™ Soft Release Avidin Resin, detected proteins on Western blot using Streptavidin Alkaline Phosphatase, and grew human umbilical arterial endothelial cells on Fibronectin-coated dishes. (1018)

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