Notes: Human umbilical vein endothelial cells (HUVEC) were resuspended in CaMK extraction buffer and lysed by Dounce homogenization. CaMK activity was measured using the SignaTECT^® Calcium/Calmodulin-Dependent Protein Kinase (CaMK II) Assay. (3402)

Notes: SignaTECT^® Calcium/Calmodulin-Dependent Protein Kinase (CaMKII) Assay System was used to determine CaMKII activity in mouse hippocampus lysates. (3401)

Notes: This study investigated the effect of CaMKII on store-operated calcium entry (SOCE).  To do this, Xenopus laevis oocytes were depleted of intracellular calcium stores by treating with thapsigargin for 3 hours.  The oocytes were then injected with an RNA encoding  constitutively active CaMKII.  After homogenization in 80mM β-glycerophosphate, 20mM HEPES pH 7.5, 15mM MgCl₂, 1mM DTT, 1mM sodium vanadate, 50mM NaF plus protease inhibitor cocktail, CaMKII activity was assayed using the SignaTECT Calcium/Calmodulin-Dependent Protein Kinase (CaM KII) Assay System. (2750)

Notes: The SignaTECT^® Calcium/Calmodulin-Dependent Protein Kinase (CaM KII) Assay System was used to analyze CaMKII activity in hippocampal and cortical primary cell cultures incubated with and without the CaM KII inhibitor KN-93. The results were expressed as percent activity compared to a control reaction. (2638)