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Protein Expr. Purif. 76, 154-164. HaloTag-based purification of functional human kinases from mammalian cells. 2010

Ohana, R.F., Hurst, R., Vidugiriene, J., Slater, M.R., Wood, K.V. and Urh, M.

Notes: The authors of this paper demonstrate the utility of the HaloTag® protein purification system for purifying functional proteins from mammalian cells. To this end five kinases were cloned into HaloTag® vectors, expressed in and purified from HEK293T cells. To demonstrate functionality of the purified recombinant kinases, activity was measured using the appropriate ADP-Glo™ Assay Kinase Enzyme Systems. (4145)

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