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Nat. Commun. 8, 14259. The tumour suppressor APC promotes HIV-1 assembly via interaction with Gag precursor protein. 2017

Miyakawa, K., Nishi, M., Matsunaga, S., Okayama, A., Anraku, M., Kudoh, A., Hirano, H., Kimura, H., Morikawa, Y., Yamamoto, N., Ono, A. and Ryo, A.

Notes: Adenomatous polyposis coli protein (APC) is shown to directly interact with HIV-1 Gag protein to stimulate Gag multimerization and spread of viral particles. Direct measurements of the Gag-Gag protein interaction were measured using the NanoBRET™ system. HeLa cells were co-transfected with Gag-HaloTag® and Gag-NanoLuc® expression vectors and BRET signal was monitored after 24 hours. ADC knockdown displayed a substantial decrease in viral production and Gag-Gag interaction. Together, the authors show ADC regulates Gag localization to the PM and viral packaging. (5058)

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