Notes: The PolyATtract^® System 1000 was used to isolate mRNA from mouse spleen. The researchers used 3μg of the isolated poly(A)+ RNA in reverse transcriptase reactions to make cDNAs. The cDNAs were ultimately used as templates in in vitro transcription reactions to produce biotin labeled cRNAs that were used as targets in microarray analysis experiments.  (2743)

Notes: The PolyATtract^® System 1000 was used to isolated polyA+ RNA directly from IMR90 cells infected with retroviruses expressing the oncogenic ras or an empty vector control. The isolated polyA+ RNA was used as template in RT-PCR reactions containing ³³P-αdCTP. The radiolabeled targets were then hybridized to microarrays (Genome Discoveries) for differential analysis of IMR90 fibroblast gene expression with the ras oncogene expressing retrovirus or an empty retrovirus control. (2695)

Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA directly from cultures of primary mouse astrocytes and the mouse microglia cell line BV-2. The isolated RNA was used for Northern analysis. (1275)

Notes: Authors use the PolyATtract^® 1000 System to isolate mRNA from rat myometrium and use this in quantitative RT-PCR experiments. (1220)

Notes: The PolyATtract® System 1000 was used to isolate poly(A)+ RNA directly from 5637 human bladder carcinoma cells. The isolated RNA was used for RT-PCR. (1272)

Notes: These authors used Promega's PolyATtract^® System 1000 to isolate mRNA from rabbit primary parietal cells. (0585)

Notes: Sf9 Spodoptera frugiperda cells were infected with the AcMNPV virus. Poly(A)+ RNA was isolated directly from the cells using the PolyATtract^® System 1000. The isolated RNA was used for cDNA library construction. (1401)

Notes: The PolyATtract^® System 1000 was used to isolate poly(A)+ RNA directly from myxoma virus-infected RK13 rabbit kidney cells. To obtain late viral RNAs, the RNA was isolated from the cells 16 hours after infection. For the early viral RNAs, the cells were treated with cycloheximide for 10 hours after infection prior to RNA isolation. The isolated RNA was used for Northern blot analysis with RNA probes prepared using the Riboprobe^® System. (0966)

Notes: The PolyATtract^® System 1000 was used to isolate poly A+ RNA directly from bovine retinas. The isolated RNA was used for RT-PCR amplification. (0369)

Notes: In this paper, the PolyATtract^® System 1000 was used to isolate mRNA from gastric mucosae cells isolated from rabbits. The mRNA was used for 3' RACE. (2010)

Notes: Poly A+ RNA was isolated directly from PBS-perfused mouse lungs with the PolyATtract^® System 1000. The isolate poly A+ RNA was used for quantitative two-step RT-PCR using M-MLV RNase Hminus Reverse Transcriptase as well as RNasin^® Ribonuclease inhibitor for first step cDNA synthesis. (0821)

Notes: The PolyATtract^® System 1000 is used to isolation polyA+ mRNA from pig main pulmonary artery endothelial cells. The isolated mRNA is used for non-radioactive Northern blots. (0090)

Notes: Poly(A)+ RNA was isolated directly from differentially treated rat adrenal glands using the PolyATtract® System 1000 and used for reverse transcription and differential display analysis. (1274)

Notes: The PolyATtract^® System 1000 was used to isolate polyA+ RNA from Pisium sp. plant tissue. Many details of the isolation are given in the paper. The isolated polyA+ RNA was used for RT-PCR and Northern blot analyses. (0736)

Notes: The PolyATtract^® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM^®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96^® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)