Notes: A primary cell line from rat subventricular zone which remains undifferentiated over time was developed as a model for MAPK activation. Cell were stimulated with 20 ng/ml bFGF and 20 ng/ml EGF (Promega) to activate MAPK. The MAPK pathway was inhibited with either U0126 (Promega) or  PD98059. Immunocytochemistry was performed with the Anti-ERK 1/2 pAb (1:100 dilution) to detect both active and inactive forms of MAPK proteins and with the Anti-ACTIVE™ MAPK pAb (1:100 dilution) to specifically detect the dually phosphorylated, active forms of MAPK. Cells were also immunostained with the neuron specific marker Anti-III-tubulin mAb (0.5 µg/ml). Cell proliferation was monitored with the CellTiter 96® AQueous One Solution Cell Proliferation Assay System. Apoptosis within the cell population was monitored using the DeadEnd™ Fluorometric TUNEL System. (2391)

Notes: Proliferation of HaCat and primary human keratinocytes to EGF, KGF and leptin was examined with the CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay (MTS/PES). (0012)

Notes: The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the proliferation of primary myocytes derived from normal and knockout mice to serum. (0017)

Notes: The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the survival of the rat retinal microglia to P2 receptor agonists and antagonists. (0010)

Notes: The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay (MTS/PES) was used to show that TNFalpha nor EGF were toxic to cultured primary human astrocytes or U-373 MG transformed human astrocytoma cells. (0016)

Notes: The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the response of transfected CTL-EN cells (a IL-2 dependent subline of CTLL-2 cells) to IL-3. The cells were transfected with either wildtype or mutant IL-3 receptor subunits. (0014)

Notes: The CellTiter 96^® AQ_ueous One Solution Assay was used to look at the differential proliferation of normal and knockout isoprenylcysteine carboxyl methyltransferase cells. The normal cells proliferated at a greater rate. (2485)

Notes: The CellTiter 96^® AQueous One Solution Assay was used to look at the differential proliferation of normal and knockout isoprenylcysteine carboxyl methyltransferase cells. The normal cells proliferated at a greater rate. (2898)

Notes: In this study, the authors document a hepatic effect for the C-type natriuretic peptide, CNP. Natriuretic peptide family members include atrial natriuretic peptide (ANP) and brain natriuretic peptide. These peptides are implicated in maintaining blood pressure, and they also exert natriuretic and diuretic effects. (2530)

Notes: The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the viability of mouse peritoneal macrophages from both wild type and p50–/– mice in response to exposure to silica. (1324)

Notes: The authors created knock out mice, deficient in the gene encoding L-isoaspartate O-methyltransferase in order to determine the biological function of this enzyme. Fibroblasts were obtained from knockout and wildtype mouse embryos and cultured. The cell viability of the cultured fibroblasts was determined using the CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay. (2490)

Notes: This paper investigated the role of  group IIA phospholipase A₂ (PLA₂) in apoptosis and sought to identify its subcellular target. Baby hamster kidney fibroblasts (BHK) were transfected with wildtype or mutant rat PLA₂, subjected to growth factor deprivation, and were assayed for survival.  Cells expressing either no PLA₂ or mutant PLA₂ underwent dramatic apoptosis; cells expressing the wildtype PLA₂ showed resistance to apoptosis. The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay was used to determine cell viability of the BHK cells. (2481)

Notes: The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay was used to assess the survival of COS-7 cells transfected with the leutinizing hormone-releasing hormone receptor. Seventy-two hours after exposure to cytotoxic compounds the cell viability was measured in relation to control cultures. (1253)

Notes: In this paper, the CellTiter 96® AQ_ueous One Solution Cell Proliferation Assay was used to assess the effects of chemicals that donate nitric oxide (NO) on proliferation of the keratinocyte cell line (HaCaT). Cells were cultured for 24hr with the chemicals prior to analysis. (0329)

Notes: The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay was used to measure the proliferative response of BAEC cells to various concentrations of vascular endothelial cell growth factor (VEGF). The cells were also cultured in the presence of the tyrosine kinase inhibitor herbimycin A or the PKC inhibitor bisindolylmaleimide. Both inhibitors blocked the VEGF-induced proliferation. (1313)

Notes: The MTS-based CellTiter 96^® AQueous One Solution Assay was used to monitor the viability of human umbilical vein epithelial cells and bovine aortic endothelial cells following treatment with various pharmacological reagents. (1728)

Notes: The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay was used to assess the proliferation of chicken granulosa layer explants in response to EGF. (0229)

Notes: The MTS-based CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay was used to quantify the anti-proliferative properties of a Raf-1 antisense oligonucleotide. Human coronary artery smooth muscle cells were grown to 80% confluency in 96 well plates and growth-arrested with medium containing no serum or growth factors. The cells were transfected with the antisense oligonucleotides and then media containing 5% serum added. Cells were assayed for various times (24-96 hours) and with various concentrations of antisense oligonucleotide. Promega’s Prime-A-Gene^® Labeling System also was used in this publication. (1707)

Notes: The MTS-based CellTiter 96^® AQueous One Solution Cell Proliferation Assay was used to assess the proliferative response of T-cells (20,000 per well) to mitomycin C-treated splenocyte antigen-presenting cells (10,000 per well) and Concanavalin A. T-cells isolated from the lung and spleen of normal mice were compared to transgenic mice expressing IL-4 constitutively in lung tissue. The lung T-cells from the transgenic mice proliferated in response to the antigen-presenting cells. (2091)

Notes: The CellTiter 96^® AQueous MTS Reagent Powder was used for assays to measure interferon alpha2a (IFN-alpha2a) inhibition of cytopathic effects (CPE) caused by a challenge of encephalomyocarditis (EMC) virus on WISH cells. (2097)