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Brain Res. Dev. Brain Res. 122, 97-109. Inhibition of mitogen-activated protein kinase kinase blocks proliferation of neural progenitor cells. 2000

Learish, R.D., Bruss, M.D., and Haak-Frendscho, M.

Notes: A primary cell line from rat subventricular zone which remains undifferentiated over time was developed as a model for MAPK activation. Cell were stimulated with 20 ng/ml bFGF and 20 ng/ml EGF (Promega) to activate MAPK. The MAPK pathway was inhibited with either U0126 (Promega) or  PD98059. Immunocytochemistry was performed with the Anti-ERK 1/2 pAb (1:100 dilution) to detect both active and inactive forms of MAPK proteins and with the Anti-ACTIVE™ MAPK pAb (1:100 dilution) to specifically detect the dually phosphorylated, active forms of MAPK. Cells were also immunostained with the neuron specific marker Anti-III-tubulin mAb (0.5 µg/ml). Cell proliferation was monitored with the CellTiter 96® AQueous One Solution Cell Proliferation Assay System. Apoptosis within the cell population was monitored using the DeadEnd™ Fluorometric TUNEL System. (2391)

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J. Clin. Invest. 106(4), 501-509. Leptin enhances wound re-epithelialization and constitutes a direct function of leptin in skin repair. 2000

Frank, S. , Stallmeyer, B. , Kampfer, H. , Kolb, N. , and Pfeilschifter, J.

Notes: Proliferation of HaCat and primary human keratinocytes to EGF, KGF and leptin was examined with the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES). (0012)

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Proc. Natl. Acad. Sci. USA 97(10), 5416-5421. Myogenic stem cell function is impaired in mice lacking the forkhead/winged helix protein MNF. 2000

Garry, D. J. , Meeson, A. , Elterman, J. , Zhao, Y. , Yang, P. , Bassel Duby, R. , and Williams, R. S.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the proliferation of primary myocytes derived from normal and knockout mice to serum. (0017)

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Neurosci. Lett. 282(3), 153-156. P2 Purinoceptor expression and functional changes of hypoxia-activated cultured rat retinal microglia. 2000

Morigiwa, K. , Quan, M. , Murakami, M. , Yamashita, M. , and Fukuda, Y.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the survival of the rat retinal microglia to P2 receptor agonists and antagonists. (0010)

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Brain Res. 868(2), 230-240. Parathyroid hormone-related protein is expressed by transformed and fetal human astrocytes and inhibits cell proliferation. 2000

Shankar, P. P. , Wei, H. , Davee, S. M. , and Funk, J. L.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to show that TNFalpha nor EGF were toxic to cultured primary human astrocytes or U-373 MG transformed human astrocytoma cells. (0016)

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Mol. Cell 6(1), 99-108. Site-specific serine phosphorylation of the IL-3 receptor is required for hemopoietic cell survival. 2000

Guthridge, M. A. , Stomski, F. C. , Barry, E. F. , Winnall, W. , Woodcock, J. M. , McClure, B. J. , Dottore, M. , Berndt, M. C. , and Lopez, A. F.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the response of transfected CTL-EN cells (a IL-2 dependent subline of CTLL-2 cells) to IL-3. The cells were transfected with either wildtype or mutant IL-3 receptor subunits. (0014)

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J. Biol. Chem. 275, 17605-17610. Targeted inactivation of the isoprenylcysteine carboxyl methyltransferase gene causes mislocalizatioin of K-Ras in mammalian cells 2000

Bergo, M.O., Leung, G.K., Ambroziak, P. Otto, J.C., Casey, P.J., and Young, S.G.

Notes: The CellTiter 96® AQueous One Solution Assay was used to look at the differential proliferation of normal and knockout isoprenylcysteine carboxyl methyltransferase cells. The normal cells proliferated at a greater rate. (2485)

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J. Biol. Chem. 275(23), 17605-17616. Targeted inactivation of the isoprenylcysteine carboxyl methyltransferase gene causes mislocalization of K-Ras in mammalian cells. 2000

Bergo, M.O., Leung, G.K., Ambroziak, P., Otto, J.C., Casey, P.J., Young, SG.

Notes: The CellTiter 96® AQueous One Solution Assay was used to look at the differential proliferation of normal and knockout isoprenylcysteine carboxyl methyltransferase cells. The normal cells proliferated at a greater rate. (2898)

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J. Biol. Chem. 274, 23761-23769. Biological effects of c-type natriuretic peptide in human myofibroblastic hepatic stellate cells 1999

Tao, J., Mallat, A., Gallois, C., Belmadani, S., Mery, P-F., Nhiew, J. T-V., Pavoine, C., Lotersztajn, S.

Notes: In this study, the authors document a hepatic effect for the C-type natriuretic peptide, CNP. Natriuretic peptide family members include atrial natriuretic peptide (ANP) and brain natriuretic peptide. These peptides are implicated in maintaining blood pressure, and they also exert natriuretic and diuretic effects. (2530)

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J. Biol. Chem. 274, 35591-35595. Involvement of 5'-flanking kappaB-like sites within bcl-x gene in silica-induced Bcl-x expression 1999

Chen, F., Demers, L.M., Vallyathan, V., Lu, Y., Castranova, V., Shi, X.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the viability of mouse peritoneal macrophages from both wild type and p50–/– mice in response to exposure to silica. (1324)

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J. Biol. Chem. 274, 20671-20678. Phenotypic analysis of seizure-prone mice lacking L-isoaspartate (D-aspartate) O-methyltransferase 1999

Kim, E., Lowenson, J.D., Clarke, S., Young, S.G.

Notes: The authors created knock out mice, deficient in the gene encoding L-isoaspartate O-methyltransferase in order to determine the biological function of this enzyme. Fibroblasts were obtained from knockout and wildtype mouse embryos and cultured. The cell viability of the cultured fibroblasts was determined using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (2490)

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J. Biol. Chem. 6, 150-155. Secretory group IIA phospholipase A2 generates anti-apoptotic survival signals in kidney fibroblasts 1999

Zhang, Y., Lemasters, J., Herman, B.

Notes: This paper investigated the role of  group IIA phospholipase A2 (PLA2) in apoptosis and sought to identify its subcellular target. Baby hamster kidney fibroblasts (BHK) were transfected with wildtype or mutant rat PLA2, subjected to growth factor deprivation, and were assayed for survival.  Cells expressing either no PLA2 or mutant PLA2 underwent dramatic apoptosis; cells expressing the wildtype PLA2 showed resistance to apoptosis. The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to determine cell viability of the BHK cells. (2481)

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Proc. Natl. Acad. Sci. USA 96, 669-673. Selective induction of apoptosis by the cytotoxic analog AN-207 in cells expressing recombinant receptor for leuteinizing hormone-releasing hormone. 1999

Danila, D.C., Schally, A.V., Nagy, A., Alexander, J.M.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to assess the survival of COS-7 cells transfected with the leutinizing hormone-releasing hormone receptor. Seventy-two hours after exposure to cytotoxic compounds the cell viability was measured in relation to control cultures. (1253)

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J. Invest. Dermatol. 113, 1090-1098. The function of nitric oxide in wound repair: inhibition of inducible nitric oxide-synthase severely impairs wound reepithelialization. 1999

Stallmeyer, B., Kampfer, H., Kolb, N., Pfeilschifter, J., Frank, S.

Notes: In this paper, the CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to assess the effects of chemicals that donate nitric oxide (NO) on proliferation of the keratinocyte cell line (HaCaT). Cells were cultured for 24hr with the chemicals prior to analysis. (0329)

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Am. J. Physiol. 277, H2038-H2049. VEGF stimulates tyrosine phosphorylation of β-catenin and small-pore endothelial barrier dysfunction. 1999

Cohen, A. W. , Carbajal, J. M. , Schaeffer, R. C. Jr

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to measure the proliferative response of BAEC cells to various concentrations of vascular endothelial cell growth factor (VEGF). The cells were also cultured in the presence of the tyrosine kinase inhibitor herbimycin A or the PKC inhibitor bisindolylmaleimide. Both inhibitors blocked the VEGF-induced proliferation. (1313)

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Blood 91(5), 1625-1632. A proteasome inhibitor, an antioxidant, or a salicylate, but not a glucocorticoid, blocks constitutive and cytokine-inducible expression of P-selectin in human endothelial cells. 1998

Xia, L., Pan, J., Yao, L., and McEver, R. P.

Notes: The MTS-based CellTiter 96® AQueous One Solution Assay was used to monitor the viability of human umbilical vein epithelial cells and bovine aortic endothelial cells following treatment with various pharmacological reagents. (1728)

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Biol. Reprod. 59, 522-526. Epidermal growth factor in the germinal disc and its potential role in follicular development in the chicken. 1998

Volentine, K.K., Yao, H.H.-C., Bahr, J.M.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to assess the proliferation of chicken granulosa layer explants in response to EGF. (0229)

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Mol. Pharmacol. 53 (1), 97-104. Exposure of human vascular smooth muscle cells to Raf-1 antisense oligodeoxynucleotides: cellular responses and pharmacodynamic implications. 1998

Schumacher, C., Cioffi, C. L., Sharif, H., Haston, W., Monia, B. P., and Wennogle, L.

Notes: The MTS-based CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to quantify the anti-proliferative properties of a Raf-1 antisense oligonucleotide. Human coronary artery smooth muscle cells were grown to 80% confluency in 96 well plates and growth-arrested with medium containing no serum or growth factors. The cells were transfected with the antisense oligonucleotides and then media containing 5% serum added. Cells were assayed for various times (24-96 hours) and with various concentrations of antisense oligonucleotide. Promega’s Prime-A-Gene® Labeling System also was used in this publication. (1707)

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Am. J. Respir. Cell Mol. Biol. 17(5), 541-51. Interleukin-4 alters epithelial cell differentiation and surfactant homeostasis in the postnatal mouse lung. 1997

Jain-Vora, S., Wert, S.E., Temann, U.A., Rankin, J.A. and Whitsett, J.A.

Notes: The MTS-based CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to assess the proliferative response of T-cells (20,000 per well) to mitomycin C-treated splenocyte antigen-presenting cells (10,000 per well) and Concanavalin A. T-cells isolated from the lung and spleen of normal mice were compared to transgenic mice expressing IL-4 constitutively in lung tissue. The lung T-cells from the transgenic mice proliferated in response to the antigen-presenting cells. (2091)

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J. Interferon and Cytokine Res. 16(1), 31-33. MTS interferon assay: A simplified cellular dehydrogenase assay for interferon activity using a water-soluble tetrazolium salt. 1996

Khabar, K.S.A., Al-Zoghaibi, F., Dzimiri, M., Taha, M., Al-Tuwaijri, A. and Al-Ahdal, M.N.

Notes: The CellTiter 96® AQueous MTS Reagent Powder was used for assays to measure interferon alpha2a (IFN-alpha2a) inhibition of cytopathic effects (CPE) caused by a challenge of encephalomyocarditis (EMC) virus on WISH cells. (2097)

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