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Am. J. Physiol. Lung Cell. Mol. Physiol. 286(4), L866-76. Effects of sham air and cigarette smoke on A549 lung cells: implications for iron-mediated oxidative damage. 2004

Mayo, J.J., Kohlhepp, P., Zhang, D., Winzerling, J.J.

Notes: A549 cells were grown to 80% confluence before being exposed to smoke or sham air for 4, 6 or 22 hours. Cells were harvested by trypsinization and viability was determined using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (3165)

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Cancer Res. 64(13), 4637-47. Prolonged extracellular signal-regulated kinase 1/2 activation during fibroblast growth factor 1- or heregulin beta1-induced antiestrogen-resistant growth of breast cancer cells is resistant to mitogen-activated protein/extracellular regulated kinase kinase inhibitors. 2004

Thottassery, J.V., Sun, Y., Westbrook, L., Rentz, S.S., Manuvakhova, M., Qu, Z., Samuel, S., Upshaw, R., Cunningham, A., Kern, F.G.

Notes: Subconfluent ML20 cells were stripped of estrogens over three days then plated in a 96-well plate at 5000 cells/well. After overnight attachment, the cells were treated with fresh media containing 5% FBS plus ICI 182780 with or without each of the following: heregulin, epidermal growth factor; fibroblast growth factor 1 and β-estradiol or each of the factors individually. Under these various media conditions, the cells were treated with U0126. After five days, the cells were assayed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (3154)

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Biomaterials 25, 843–850. Protection of insulin secreting cells from nitric oxide induced cellular damage by crosslinked hemoglobin. 2004

Chaea, S.Y., Leeb, M., Kimb, S.W. and Baeb, Y.H.

Notes: The authors studied the effect of nitric oxide stress on rat Islets of Langerhans cells and rat insulinoma cell line (RINm5F) and to determine if cross-linked hemoglobin (Hb-C)could mediate the stress and subsequent apoptosis.  The rat cells were treated with a nitric oxide donor SNAP and the DeadEnd™ Colorimetric TUNEL System was used to measure the effect of NO with or without Hb-C.  In addition, the cell viability was assessed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay.  Twenty-four hours after SNAP treatment, the level of NO2 (a by-product of NO) was measured using Griess Reagent to determine the level of nitric oxide production induced. (3053)

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J. Physiol. 558(Pt 1), 181-91. Response of human cells to desiccation: comparison with hyperosmotic stress response. 2004

Huang, Z., Tunnacliffe, A.

Notes: Cells were plated in a 96-well plate and incubated until near confluence. The cells were subsequently dried by removing the media before being placed in a humid atmosphere for 2-24 hours. Then the cells were rehydrated by incubating in media for two hours before being assayed for cell viability using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (3163)

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Mol. Cell. Biol. 24(7), 2853-62. Silencing of chromatin assembly factor 1 in human cells leads to cell death and loss of chromatin assembly during DNA synthesis. 2004

Nabatiyan, A., Krude, T.

Notes: HeLa cells were grown in 24-well plates and transfected with nontarget and p60 siRNAs with untransfected cells as a control. After 24 and 48 hours, loss of cell viability upon silencing of p60 was assessed by incubating the cells with the CellTiter 96® AQueous One Solution Cell Proliferation Assay for five minutes and then stopping the reaction. (3156)

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J. Invest. Dermatol. 123(2), 380-7. The novel synthetic oleanane triterpenoid CDDO (2-cyano-3, 12-dioxoolean-1, 9-dien-28-oic acid) induces apoptosis in Mycosis fungoides/Sezary syndrome cells. 2004

Zhang, C., Ni, X., Konopleva, M., Andreeff, M., Duvic, M.

Notes: Aliquots of 5 x 104 cells/well were distributed in 96-well plates and incubated with 1, 2, and 5 µM novel synthetic oleanane triterpenoid CDDO for 48 hours. Then the relative cell viability was determined by comparing untreated control (DMSO) to treated groups using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (3164)

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Clin. Can. Res. 10(6), 1901-10. Transendothelial migration of myeloma cells is increased by tumor necrosis factor (TNF)-alpha via TNF receptor 2 and autocrine up-regulation of MCP-1. 2004

Johrer, K., Janke, K., Krugmann, J., Fiegl, M., Greil, R.

Notes: 0.5 x 106 cells/ml were seeded in 96-well plates and incubated for 24 or 48 hours with or without recombinant human TNF-α or recombinant human MCP-1. The CellTiter 96® AQueous One Solution Cell Proliferation Assay was added to the cells and incubated for 4 hours before the absorbance was measured. (3157)

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Assay Drug Dev. Technol. 2, 51-62. Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. 2004

Riss, T.L. and Moravec, R.A.

Notes: The effects of tamoxifen and vinblastine were assayed on HepG2 and HL-60 cells using a variety of Promega’s cell-based assays. The CellTiter-Glo® Luminescent Cell Viability Assay, CellTiter 96® AQueous One Solution Cell Proliferation Assay, and CellTiter-Blue® Cell Viability Assay were used to test the effects of 0-100µM tamoxifen on HepG2 cell viability after an incubation period ranging from 0-24 hours. Cell membrane integrity was assessed with the CytoTox-ONE™ Homogeneous Membrane Integrity Assay. The Caspase-Glo® 3/7 Assay was also used to measure Caspase 3/7 activity after 0-100µM tamoxifen treatment. (3189)

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Clin. Can. Res. 9, 2933-2939. Expression of constitutively active Akt-3 in MCF-7 breast cancer cells reverses the estrogen and tamoxifen responsivity of these cells in vivo 2003

Faridi, J., Wang, L., Endermann, G., Roth, R.A.

Notes: MCF-7 cells were transfected with a plasmid encoding constitutively active Akt-3. To assess estrogen-related transcriptional activity, the cells were transfected with an ERE-luciferase (firefly) reporter and a Renilla luciferase control plasmid. Dual luciferase assays were performed to determine the effect of estrogen on transcription in these Akt-3 expressing cells. Additionally, proliferation assays were performed using the CellTiter® AQueous One Solution Cell Proliferation Assay and showed that the Akt-3 expression confers serum-independent growth on the cells. (2709)

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Assay Drug Dev. Technol. 3, 469-477. HTS Compatible Assay for Antioxidative Agents Using Primary Cultured Hepatocytes 2003

Gaunitz, F. and Heise, K.

Notes: Primary hepatocytes from Sprauge-Dawley rats were incubated with various concentrations of tert-butylhydroperoxide to induce oxidative stress and then cell viability, cytotoxicity and apoptosis were assayed with Promega's CellTiter 96 AQueous One Solution Cell Proliferation Assay, CellTiter-Blue Cell Viability Assay, CellTiter-Glo Luminescent Cell Viability Assay, CytoTox-ONE Homogeneous Membrane Integrity Assay, Apo-ONE Homogeneous Caspase-3/7 Assay and assay systems. (2969)

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Clin. Can. Res. 9, 1528-1534. Possible role of placental leucine aminopeptidase in the antiproliferative effect of oxytocin in human endometrial adenocarinoma 2003

Suzuki, Y., Shibata, K., Kikkawa, F., Kajiyama, H., Kazuhiko, I., Nomura, S., Tsujumoto, M., Mizutani, S.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay  was used to evaluate the effect of oxytocin on proliferation of human endometrial  endometriod adenocarcinoma cell lines. (2654)

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J. Biol. Chem. 278 (52), 52307-52314. Silencing of RNA helicase II/Gu inhibits mammalian ribosomal RNA production. 2003

Henning, D., So, R.B., Jin, R., Lau, L.F. and Valdez, B.C.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to analyze the effect of siRNA transfection on HeLa cell proliferation. HeLa cells were transfected twice with 10nM of a siRNA for RNA helicase II/Guα, a scrambled sequence siRNA, or mock transfected with the transfection reagent. Transfected cells (50,000-100,000) were seeded in 6-well culture plates for 72 hours. After this incubation, cells were plated at 625-20,000 cells per well in 96-well culture plates, and were then assayed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay.  (3036)

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Clin. Can. Res. 8, 1234-1240. Induction of apoptosis by bexarotene in cutaneous T-Cell lymphoma cells: Relevance to mechanism of therapeutic action 2002

Zhang, C., Hazarika, P., Ni, X., Weidner, D., Duvic, M.

Notes: The authors investigated the mechanism of action of the synthetic rexinoid, bexarotene, in the treatment of cutaneous T-Cell Lymphoma (CTCL). They used the CellTiter 96® AQueous One Solution Cell Proliferation Assay to assess the effect of bexarotene on three human CTCL cell lines (MJ, Hut78 and HH). Cells were treated with or without 0.1, 1, or 10μM bexarotene for varying time periods. Results indicated significant inhibition of cell growth as dosage increased. (2448)

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Br. J. Cancer 87, 98-105. Novel substituted methylenedioxy lignan suppresses proliferation of cancer cells by inhibiting telomerase and activation of c-myc and caspases leading to apoptosis. 2002

Giridharan, P., Somasundaram, S.T., Perumal, K., Vishwakarma, R.A., Karthikeyan, N.P., Velmurugan, R., and Balakrishnan, A.

Notes: In this paper, the CellTiter 96® Aqueous Assay was used to assess cell proliferation. (2525)

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Nature 418, 386. Screening inhibitors of anthrax lethal factor. 2002

Tonello, F., Seweso, M., Marin, O., Mock, M., and Montecucco, C.

Notes: This brief communication discusses substrates of anthrax lethal factor that can be used for high-throughput screening of potential inhibitors. The CellTiter® Aqueous Cell Proliferation Assay was used to assess the effect of selected inhibitors on cytotoxicity of lethal factor in RAW264.7 cells. (2555)

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Clin. Can. Res. 8, 2448-2454. Selective inhibition of epidermal growth factor receptor by ZD1839 decreases the growth and invasion of ovarian clear cell adenocarcinoma cells 2002

Fujimura, M. Hidaka, T. and Shigeru, S.

Notes: The authors investigated the effect of several growth factors that bind tyrosine kinase receptors on the growth and invasion of three ovarian clear cell carcinoma cell lines. They used the CellTiter® AQueous One Solution Cell Proliferation Assay to measure cell proliferation after treatments. The epidermal growth factor ZD1839 showed inhibitory effects. (2496)

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Mol. Cell. Biol. 2001, 8385-8397. Neuropeptide-induced androgen independence in prostate cancer cells: Roles of noreceptor tyrosine kinases Etk/Bmx, Src, and Focal Adhesion Kinase 2001

Lee, L-F., Guan, J., Qiu, Y., Kung, H-J.

Notes: The authors investigated the inhibitory effect of flutamide on bombesin-induced cell proliferation in LNCaP prostate epithelial cells. Cells were preincubated in the presence or absence of flutamide and then treated with either R1881 (control) or bombesin for 72 hours under charcoal-stripped serum conditions. Cell proliferation was measured using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (2527)

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J. Neurochem. 74, 2278-2287. 4-Hydroxynonenal induces oxidative stress and death of cultured spinal cord neurons 2000

Malecki, A., Garrido, R., Mattson, M.P., Hennig, B., Toborek, M.

Notes: The authors treated mouse spinal cord neurons with different doses of 4-hydroxynonenal (HNE) and assessed cytotoxicity using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (2533)

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J. Biol. Chem. 275, 9095-9098. Activated protein C directly activates human endothelial gelatinase A. 2000

Nguyen, M. , Arkell, J. , Jackson, C.J.

Notes: In this paper, the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to normalize data to the number of cells present in each well. Equal numbers of cells were grown in each well and the conditioned media removed for enzymatic assays. The relative number of human umbilical vein endothelial cells remaining were measured with the assay. (0608)

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J. Immunol. 165(5), 2500-2510. Apoptosis induced by TGF-beta 1 in Burkitt's lymphoma cells is caspase 8 dependent but is death receptor independent. 2000

Inman, G. J. , and Allday, M. J.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the ability of different soluble receptors to block apoptotic agents. A soluble DR5/Fc fusion blocked TRAIL-mediated apoptosis but not TGFbeta1-mediated apoptosis. (0011)

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J. Biol. Chem. 275, 8772-8778. Chaperones Hsp70 and Hsp40 suppress aggregate formation and apoptosis in cultured neuronal cells expressing truncated androgen receptor protein with expanded polyglutamine tract. 2000

Kobayashi, Y., Kume, A., Li, M., Doyu, M., Hata, M., Ohtsuka, K., Sobue, G.

Notes: The DeadEnd™ Colorimetric Apoptosis Detection System was used to detect apoptosis in transfected Neuro 2a cells. The cells were transfected with truncated androgen receptors that aggregate in either the nuclei or the cytoplasm. The cells with nuclear aggregates were apoptotic and those with cytoplasmic aggregates were mostly nonapoptotic. The location of the aggregates was determined with GFP fusions. The basic protocol of the DeadEnd™ Colorimetric Apoptosis Detection System was modified to use streptavidin-Texas Red® for fluorometric detection rather than colorimetric detection. Good detail is provided for the modifications. Neuro 2a cells expressing the aggregating AR mutations as well as various chaperonins were analyzed for cell viability at 24, 48 and 72 hours with the CellTiter 96® AQueous One Solution (MTS/PES). The name of the DeadEnd™ Colorimetric Apoptosis Detection System has been changed to DeadEnd™ Colorimetric TUNEL System. (0913)

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FASEB J. 14, 1801-1813. Definition of endotoxin binding sites in horseshoe crab Factor C recombinant sushi proteins and neutralization of endotoxin by sushi peptides. 2000

Tan, N.S., Ng, M.L., Yau, Y.H., Chong, P.K., Ho, B. and Ding, J.L.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to assess toxicity of peptides in cell culture. The cells were THP-1 monocytes, and the peptide concentrations used were 1.25 to 320µM. (2127)

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Brain Res. 857, 265-274. Glial growth factor 2 induces proliferation and structural changes in ensheathing cells. 2000

Chuah, M. I. , Cossins, J. , Woodhall, E. , Tennent, R. , Nash, G. , and West, A. K.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to examine the proliferation of rat olfactory bulb ensheathing cells to glial growth factor 2. (0009)

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FASEB J. 14, 859-870. High-affinity LPS binding domain(s) in recombinant factor C of a horseshoe crab neutralizes LPS-induced lethality 2000

Tan, N.S., Ho, B., Ding, J.L.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to measure the cytotoxic effect of a biologically active, recombinant fragment of factor C. Factor C is an endotoxin-sensitive serine protease. Various concentrations of the factor C fragment were added to THP-1 cells and allowed to react for 60 minutes prior to the assay. (2545)

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Neurosci. Lett. 288(1), 37-40. Induction of rat L-phosphoserine phosphatase by amyloid-beta (1-42) is inhibited by interleukin-11. 2000

Heese, K. , Nagai, Y. , and Sawada, T.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to judge the percent survival of B104 rat neuroblastoma cells in response to beta-amyloid peptides as well as glutamate and serine combinations. (0015)

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