Leite, F., O’Brien, S., Sylte, M.J., Page, T., Atapattu, D. and Czuprynski, C.J.
Notes: Promega’s Human Recombinant Tumor Necrosis Factor-α (rhTNF-α) was added to bovine peripheral blood neutrophils (PMN) to induce expression of lymphocyte function-associated antigen 1 (LFA-1). Researchers incubated 1 x 106 cultured bovine PMNs with 50ng of rhTNF-α for 15 or 60 minutes. LFA-1 was detected by flow cytometry. The authors also used the Colorimetric CaspACE™ Assay System to assess apoptosis in bovine PMNs. For these studies, protein normalized cell lysates were used to compare caspase-3 activity to LFA-1 expression. (2699)