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Infect. Immun. 70, 4336-4343. Inflammatory cytokines enhance the interaction of Mannheimia haemolytica leukotoxin with bovine peripheral blood neutrophils in vitro. 2002

Leite, F., O’Brien, S., Sylte, M.J., Page, T., Atapattu, D. and Czuprynski, C.J.

Notes: Promega’s Human Recombinant Tumor Necrosis Factor-α (rhTNF-α) was added to bovine peripheral blood neutrophils (PMN) to induce expression of lymphocyte function-associated antigen 1 (LFA-1). Researchers incubated 1 x 106 cultured bovine PMNs with 50ng of rhTNF-α for 15 or 60 minutes.  LFA-1 was detected by flow cytometry. The authors also used the Colorimetric CaspACE™ Assay System to assess apoptosis in bovine PMNs. For these studies, protein normalized cell lysates were used to compare caspase-3 activity to LFA-1 expression. (2699)

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Am. J. Physiol. 273, E1052-E1058. Leucine metabolism in TNF-α- and endotoxin-treated rats: contribution of hepatic tissue. 1997

Holecek, M., Sprongl, L., Skopec, F., Andrys, C., Pecka, M.

Notes: The authors injected Tumor Necrosis Factor-α (TNF-α) into rats to study its effect on whole body leucine metabolism. (1054)

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Proc. Natl. Acad. Sci. USA 94, 10057-10062.. Suppression of tumor necrosis factor-induced cell death by inhibitor of apoptosis c-IAP2 is under NF-kappaB control 1997

Chu, D. M. , McKinsey T. A. , Liu L. , Gentry J. J. , Malim M. H. , Ballard D. W.

Notes: Tumor Necrosis Factor-alpha (TNF-alpha) was used to stimulate Jurkat cells in culture. Total RNA and nuclear extracts were prepared from the stimulated cells. Reporter activity was also analyzed. (1301)

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