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EMBO J. 16, 1080-1092. Tumor necrosis factor alpha-induced activation of c-jun N-terminal kinase is mediated by TRAF2. 1997

Reinhard, C., Shamoon, B., Shyamala, V., Williams, L.T.

Notes: A firefly luciferase reporter vector containing three copies of the Ig kappa NF-kB binding site was cotransfected with vectors expressing either TRAF2 with or without MAPK Kinase 4 or mutants of TRAF2. All firefly luciferase activity was normalized to Renilla luciferase activity provided by the pRL-TK Vector. These studies were performed in COS 7 cells. Luciferase activities were determined with the Dual-Luciferase® Reporter Assay System. (0508)

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J. Biol. Chem. 272, 30208-30214. Two naturally occurring insulin receptor tyrosine kinase domain mutants provide evidence that phosphoinositide 3-kinase activation alone is not sufficient for the mediation of insulin's metabolic and mitogenic effects. 1997

Krook, A., Whitehead, J.P., Dobson, S.P., Griffiths, M.R., Ouwens, M., Baker, C., Hayward, A.C., Sen, S.K., Maassen, J.A., Siddle, K., Tavaré, J.M., O'Rahilly, S.

Notes: CHO-K1 cells were transfected at ~80% confluency with four plasmids: (1) a CMV-driven vector expressing the wildtype or mutant human insulin receptor; (2) a pGL3-Basic-derived Vector containing five GAL4 binding sites upstream of the firefly luciferase reporter; (3) the pRL-CMV Control Vector; and (4) a fusion of the GAL4-binding domain and Elk-1 transcription factor. The Dual-Luciferase® Reporter Assay System was used to confirm that specific mutations in the insulin receptor negate its ability to activate the Elk-1 transcription factor. (0896)

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J. Biol. Chem. 272, 17097-17103. Vasoactive peptides modulate vascular endothelial cell growth factor production and endothelial cell proliferation and invasion 1997

Pedram, A., Razandi, M., Hu, R. M., Levin, E. R.

Notes: Vascular smooth muscle cells were transfected with a firefly luciferase reporter vector and pRL-SV40 as a control. Transfection efficiency was normalized to the Renilla luciferase activity as determined by the Dual-Luciferase® Reporter Assay System. (0555)

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