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SLAS Discov 24(7):745-754, 745-754. Evaluation of Luminogenic Substrates as Probe Substrates for Bacterial Cytochrome P450 Enzymes: Application to Mycobacterium tuberculosis. 2019

Ortega Ugalde, S., Ma, D., Cali, J.J., and Commandeur, J.N.M.

Notes: Pro-luciferin substrates were evaluated as substrates for Mycobacterium tuberculosis cytochrome P450 enzymes to support applicability for high-throughput screening. (5206)

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Lab. Invest. 98(11), 1500-1511. Establishment and analysis of a mouse model that regulates sex-related differences in liver drug metabolism. 2018

Chikada, H., Ida, K., Ando, E., Inagaki, Y., Sakamoto, A., and Kamiya, A.

Notes: Gene expression profiles in males and females, related to cytochrome P450s, were evaluated in a mouse model. The activity of CYP2B6 in adult hepatocytes differed between the sexes.  (5213)

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P450-Glo™ CYP2B6 Assay

J. Pharmacol. Toxicol. Methods 92, 77-94. mRNA transfection retrofits cell-based assays with xenobiotic metabolism. 2018

DeGroot, D.E., Swank, A., Thomas, R.S., Strynar, M., Lee, M.Y., Carmichael, P.L., and Simmons, S.O.

Notes: The ten most common human liver cytochrome P450 enzymes were introduced into a HEK293T cell line to support the creation of high throughput screening assays. Luminogenic substrates for the P450 enzymes were used to assess activity. (5212)

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P450-Glo™ CYP1A2 Assay

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18, 1085. Assembly of Hepatocyte Spheroids Using Magnetic 3D Cell Culture for CYP450 Inhibition/Induction. 2017

Desai, P. K., Tseng, H. and Souza, G. R. 

Notes: CYP450 activity was measured using the P450-Glo™ Assays for CYP3A4, CYP2B6 and CYP1A2, and viability was measured using two separate luminescent assays, one of which being CellTiter-Glo® Cell Viability Assay. (4877)

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CellTiter-Glo® Luminescent Cell Viability Assay

P450-Glo™ CYP3A4 Assay with Luciferin-IPA

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