Notes: Human colon cancer (HT29-D4) cells were analyzed for activated caspases using the CaspACE™ FITC-VAD-FMK In Situ Marker. HT29-D4 cells (7 x 10⁵) were cultured in the presence or absence of 1µM orexins, peptide growth inhibitors. Cells were washed, and bound CaspACE™ FITC-VAD-FMK In Situ Marker was visualized by confocal microscopy. (3255)

Notes: Apoptosis in yeast cells was detected using the CaspACE™ FITC-VAD-FMK In Situ marker. Yeast cells were stained with the marker at room temperature, washed and resuspended. FACS analysis of cells was performed with excitation at 488nm and emission of 520-550nm. (2657)

Notes: The CaspACE™ FITC-VAD-FMK In Situ Marker was used at a concentration of 5mM in primary human epidermal keratinocyte culture to visualize active caspases upon differentiation with calcium. In this experiment, the authors cultured primary human epidermal keratinocytes for 48 hours in 1.2mM calcium with or without 100mM z-VAD-FMK to demonstrate specific caspase activation and cell differentiation in calcium induced keratinocytes upon labeling with the CaspACE™ FITC-VAD-FMK In Situ Marker.  (2691)

Notes: In this article, the CaspACE™ FITC-VAD-FMK in Situ Marker was used to stain tobacco plant cells induced to undergo apoptosis. (2557)

Notes: The CaspACE™ FITC-VAD-FMK In Situ Marker is used to determine if dendritic cells are undergoing apoptosis following incubation with 9L gliosarcoma tumor cells. Cells were analyzed using fluorescence microscopy. (2414)

Notes: In this paper the CaspACE™ FITC-VAD-FMK in situ marker was used to detect activated caspases in tumor cells. (2522)

Notes: The effects of pituitary adenylate cyclase-activating polypeptide (PACP) on ethanol induced caspace activation in cultured granule cells was measured using the CaspACE™ FITC-VAD-FMK In Situ Marker. (2416)

Notes: Tumore necrosis factor α-induced apoptosis of permanently transfected HeLa cells expressing the IG20 cDNA or its splice isoforms were assayed using the CaspACE™ FITC-VAD-FMK In Situ Marker. The activation os specific caspases was determined using the CaspACE™ Assay System. The Dual-Luciferase Assay System was used to measure TNF α-induced NF-κB activation in HeLA-IG20 cells. (2418)

Notes: The CaspACE™ FITC-VAD-FMK In Situ Marker is used to assess apoptosis in pancreatic β-cells. Cells are analyzed using flow cytometry (2413)

Notes: Cultured rat oligodendrocyte progenitor cells were infected with adenoviruses expressing either wildtype Akt or a mutant with alanines in place of Thr308 and Ser473. The cells were cultured for 24 or 48 hours and then assayed for caspase activation with the CaspACE™ FITC-VAD-FMK In Situ Marker. The positive cells were viewed under a microscope and counted. At 24 hours, the wildtype Akt and control, mock-infected cells were almost identical in percent caspase-positive cells (10.5% wildtype vs. 8.9% control) and 50% for the mutant Akt. The percentage of caspase-positive cells increases to 55% at 72 hours. (0051)

Notes: In this paper the CaspACE™ FITC-VAD-FMK marker was used to detect activated caspase in sea squirt (Ciona) tail during metamorphosis in situ. (2625)