Mondal, S., Hsiao, K., and Goueli, S.A.
Notes: These authors describe a new bioluminescent assay for monitoring GTPase activity, and demonstrate it's utility for monitoring GTPase, GAP-stimulated GTPase, GAP, and GEF activities. The system can also be used to analyze these proteins expressed in cells as fusion proteins using a pulldown assay format. The assays showed minimal false hits upon testing for compound interference using the library of pharmacologically active compounds. (4584)