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Assay Drug Dev. Technol. 13 (8), 444-455. A Homogenous Bioluminescent System for Measuring GTPase, GTPase Activating Protein, and Guanine Nucleotide Exchange Factor Activities. 2015

Mondal, S., Hsiao, K., and Goueli, S.A.

Notes: These authors describe a new bioluminescent assay for monitoring GTPase activity, and demonstrate it's utility for monitoring GTPase, GAP-stimulated GTPase, GAP, and GEF activities. The system can also be used to analyze these proteins expressed in cells as fusion proteins using a pulldown assay format. The assays showed minimal false hits upon testing for compound interference using the library of pharmacologically active compounds. (4584)

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