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Citations Search

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FASEB J. 21, 1893–1901. Low expression of COX-2, reduced cumulus expansion, and impaired ovulation in SULT1E1-deficient mice. 2007

Gershon, E., Hourvitz, A., Reikhav, S., Maman, E. and Dekel, N.

Notes: The authors investigated the role of estrogen inactivation by the SULT1E1-encoded estrogen sulfotransferase in ovulation in mice. Semiquantitative RT-PCR was used to characterize the temporal and tissue-specific expression of SULT1E1 mRNA in ovulating mice. First-strand cDNA synthesis was performed at 37°C for 2 hours using 7.5µg of total RNA, 1µl (0.5µg) of oligo(dT)15, 40 units of RNasin® Ribonuclease Inhibitor and 200 units of M-MLV Reverse Transcriptase. (3913)

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Clin. Can. Res. 9, 3080-3097. Gene expression profiling of malignant mesothelioma 2003

Singhal, S., Wiewrodt, R., Malden, L.D., Amin, K.M., Matzie, K., Friedberg, J., Kucharczuk, J.C., Lizky, L.A., Johnson, S.W., Kaiser, L.R., Albelda, S.M.

Notes: RNA was extracted from 300mg malignant mesothelioma tissue samples and treated with DNase in the presence of RNasin® Ribonuclease Inhibitor. The RNA was incubated with oligo(dT) primers and reverse transcribed in the presence of radiolabeled nucleotides. The labeled cDNA was hybridized to preprinted microarrays containing 4132 human genes. (2715)

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J. Gen. Virol. 80, 1929-1941. Sequence analysis of a porcine enterovirus serotype 1 isolate: Relationships with other picornaviruses. 1999

Doherty, M., Todd, D., McFerran, N., Hoey, E.M.

Notes: RNA was isolated from purified porcine enterovirus serotype 1 and oligo-d(T) primed, double stranded cDNA synthesis was accomplished using the Universal Riboclone® cDNA Synthesis System. The dsDNA was subcloned into the pGEM®-3Z Vector using of EcoRI Adaptors. (1239)

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