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Nucl. Acids Res. 38, 522–33. An integrated pipeline for next-generation sequencing and annotation of mitochondrial genomes 2010

Jex, A.R., Hall, R.S., Littlewood, D.T. and Gasser, R.B.

Notes: Wizard® SV Gel and PCR Clean-Up System was used to clean up genomic DNA isolated from parasitic nematodes isolated from a variety of animals. Species identification of each nematode specimen was determined via PCR amplification of specific nuclear DNA followed by purification of the amplified product using Wizard® PCR Preps DNA Purification System before sequencing using BigDye chemistry. Wizard® SV Gel and PCR Clean-Up System was also used to prepare amplicons generated by long-PCR of mt genomes from the nematodes before NGS sequencing. Results from NGS were confirmed using PCR-based sequencing of short mt DNA tracts. Short mtDNA regions were amplified by conventional PCR. Amplicons were purified using Wizard® PCR Preps DNA Purification System before sequencing using BigDye chemistry. (4533)

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J. Leukoc. Biol. 79, 202–13. Induction of intracellular calcium elevation by {Delta}9-tetrahydrocannabinol in T cells involves TRPC1 channels. 2006

Rao, G.K. and Kaminski, N.E.

Notes: The authors studied the relationship between transient receptor potential canonical (TRPC) channels and Ca2+ elevation in the cannabinoid-2 receptor-expressing human peripheral blood-acute lymphoid leukemia (HPB-ALL) human T cell line. Total RNA from HPB-ALL cells was subjected to RT-PCR and the bands for TRPC1 were excised from a 1.2% NuSieve 3:1 agarose gel, purified using the Wizard® PCR Preps DNA Purification System and sequenced. Using 20nM synthesized siRNA specific for TRPC1 and a nonsilencing control sequence, 2.5 × 105 HPB-ALL cells/ml (a human T cell line) were transiently transfected for 48 hours using the CodeBreaker™ siRNA Transfection Reagent. After this 48-hour incubation, the siRNA-treated cells were either used for calcium determination or harvested and washed, and the RNA was isolated using the SV Total RNA Isolation System. The RNA was used for quantitative real-time PCR. (3316)

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J. Virol. 78, 9936-9946. Poly(ADP-Ribose) polymerase 1 binds to Kaposi's sarcoma-associated Herpesvirus (KSHV) terminal repeat sequence and modulates KSHV replication in latency. 2005

Ohsaki, E., Ueda, K., Sakakibara, S., Do, E., Yada, K. and Yamanishi, K.

Notes: Three terminal repeat sequences (TR) from Kaposi's sarcoma-associated herpesvirus (KSHV) were PCR amplified and then purified using the Wizard® PCR Preps DNA Purification System. The PCR products were 386, 424, and 307 base pairs in size.  These TR products were used in PARP1-DNA ELISA assays.  The authors also used the Wizard® SV Genomic DNA Purification System to isolate DNA from a BC3 cell line infected with KSHV. The isolated DNA was used in real-time PCR to assess KSHV copy number in cells. (3232)

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Clin. Can. Res. 9, 1053-1056. A cancer-prone case with a background methylation of p16 tumor suppressor gene 2003

Hibi, K., Koike, M., Nakayama, H., Fujitake, S., Kasai, Y., Ito, K., Akiyama, S., Nakao, A.

Notes: DNA from human epithelium, blood, and tumor specimens was subjected to bisulfite treatment. Bisulfite-treated DNA was purified using Wizard® DNA purification resin. (2634)

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Infect. Immun. 68, 7180-7185. Phylogeny of Vibrio cholerae based on recA sequences. 2000

Stine, O.C., Sozhamannan, S., Gou, Q., Zheng, S., Morris, J.G., Jr., and Johnson, J.A.

Notes: Chromosomal DNA was isolated from V. cholerae with the Wizard® Genomic DNA Purification Kit prior to PCR.  The PCR products were directly purified with the Wizard® PCR Preps System.  The purified 788bp amplimers were used directly for fluorescent Big Dye sequencing. (2299)

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