Notes: These researchers screened for microbiome metabolites that activated inflammasomes, using cell viability and caspase-1 activity assays, and a CRISPR genome-wide screen.  (5205)

Notes: Based on emerging interest in using cellulose nanocrystals for biomedical applications, the impact of these on immunological responses was evaluated. Human and murine cell lines THP-1, J774A.1 were used with multiple readouts, including caspase-1 activation, cytokine release and cytotoxicity. (5215)

Notes: In this study, researchers evaluated viral protein activity on inflammasome activation, including measuring the activation of caspase-1 by wild-type and mutant proteins. (5214)

Notes: These authors evaluated the toxicity and the anti-inflammatory effects of various traditional herbal remedies used for asthma, with the goal of selecting promising candidates for further characterization. Remedies were tested for toxicity on THP-1 derived macrophages using the CellTiter-Glo® Luminescent Cell Viability Assay. The Caspase-Glo® 1 assay was used to evaluate inflammatory effects.

Notes: The protective effect of globular adiponectin (gAcrp) in endoplasmic reticulum (ER) stress and cell death in hepatocytes was investigated. Treatment with gAcrp decreased caspase activation and lowered lactate dehydrogenase release as measured by the Caspase-Glo® 1 and CytoTox® 96 Non-Radioactive Cytotoxicity assays. These results show that gAcrp protects the liver from inflammation. (5253)

Notes: The effects of hydrogen sulfide on inflammasome activation were investigated. Lactate dehydrogenase levels and caspase activation were monitored using the CytoTox-ONE™ and Caspase-Glo® assays. Hydrogen sulfide was shown to decrease caspase activation and inflammasome activity both in vitro and in vivo. (5147)

Notes: The researchers monitored caspase 1 activity in hypoxia models using Caspase-Glo® 1 Inflammasome Assay. (4994) (4994)

Notes: These authors investigated silicon dioxide nanoparticles (SiNPs) as a potential cancer treatment and identified the mechanism mediating cancer cell death. The Caspase-Glo® 3/7, Caspase-Glo® 9, CellTiter-Glo® luminescent cell-viability, ROS-Glo™, and Caspase-Glo® 1 assays were used to monitor cellular response to SiNPs. Together, SiNPs were identified to activate the intrinsic apoptosis pathway and lead to cell death.

  (5165)

Notes: These researchers sought compounds that could be potential leads for the development of newer caspase-1 inhibitors as anti-inflammatory agents. They used Caspase-Glo® 1 Inflammatory Assay. (4995)

Notes: These authors used the Caspase-Glo® 1 Assay in their study of 2,4-diaminopyrimidine derivatives (6a-6w) as potential caspase-1 inhibitors. (5012)

Notes: The role of oxysterol levels in skin aging and degenerative diseases was examined. Specifically, 25-OH oxysterol showed effects on inflammation and led to an increase in caspase-dependent apoptosis. Caspase levels were measured using the Caspase-Glo® 1 assay. Together, 25-OH seems to lead to toxicity and degenerative disorders. (5152)

Notes: The role of the carcinogen Ochratoxin A in cellular inflammation was examined. HEK293 cells were treated with Ochratoxin A and cellular inflammatory markers were monitored. ATP levels and caspase activation were analyzed using the CellTiter Glo® and Caspase-Glo® assays. (5155)

Notes: Immune defense mechanisms against the fungal pathogen Candida albicans were investigated using immune cell–supplemented 3D skin models. These models identified the epidermal cell types responsible for protection against C. albicans. The Caspase-Glo® 1 assay was used to show that activation of caspase-1 required the presence of T-cells. (5160)

Notes: The authors determined the impact of IL-27 on caspase-1 activity using Capase-Glo® 1 Inflammasome Assay. (4993) (4993)

Notes: A novel target of non-steroidal anti-inflammatory drugs (NSAIDs) was identified using the Caspase-Glo® -1, Caspase-Glo® 3/7, and Caspase-Glo® 9 assays. The authors observed that an inhibition of caspase activity lead to decreased cell death and inflammation. The CellTiter-Fluor™ and CellTiter-Glo®  assays were using to monitor cell viability. (5163)

Notes: Chagas disease is caused by the parasite Trypanosoma cruzi and can lead to Chronic Chagas disease cardiomyopathy (CCC). The ability of N,N-dimethylsphingosine (DMS) to decrease inflammation and function as a therapeutic for CCC was determined. As part of this study, Caspase-Glo® assays were used to detect inflammatory (caspase 1 and 8), or apoptotic (caspase 9 and 3/7) activities. (5166)

Notes: Researchers used the Caspase-Glo® 1 Inflammasome Assay to take measure Z-WEHD-aminoluciferin capase-1 substrate activity. (4827)

Notes: The authors utilized phosphoproteomics to identify host factors that are activated upon Influenza A infection. In total, 1116 proteins showed changes in regulation including cyclin-dependent kinases. The authors tested the effects of cyclin-dependent kinase inhibitors on cell viability and caspase activation using the CellTiter Glo® and Caspase-Glo® assays. (5154)

Notes: A combination therapy of the chemotherapeutic cisplatin and an IL-1R antagonist was tested as a potential treatment in malignant mesothelioma (MM). The authors observed both caspase-1 activation and pyroptosis upon treatment. Caspase activation was measured using the Caspase-Glo® 1 assay, and pyroptosis was measured with the CellTiter® 96 Aqueous One Solution Assay. (5148)

Notes: The authors investigated the signaling pathways contributing to elevated cytokine levels in patients with severe dengue virus (DENV) infection. The Caspase-Glo® 1 assay was used to measure caspase-1 levels in DENV infected monocytes. Caspase-1 was found to be required for Interleukin-1ß maturation and production.  (5164)